SARS-CoV-2 (2019-nCoV) Spike RBD Antigen Quantitative ELISA Assay Kit
The SARS-CoV-2 (2019-nCoV) Spike RBD Antigen Quantitative ELISA Assay Kit is For Research Use Only
Size: 12×8 wells
Standard Range: 15-720 ng/mL
Incubation Time: 2 hours 30 minutes
Sample Type: Cell Culture Supernatant
Sample Size: 100 µL
Alternative Name: Coronavirus, SARS-CoV, Covid-19
Assay Principle
The method employs sandwich ELISA technique. Monoclonal antibody specific for SARS-CoV-2 (2019-nCoV) Spike RBD is pre-coated onto microwells. Samples and standards are pipetted into microwells and SARS-CoV-2 (2019-nCoV) Spike RBD Antigen present in the sample is bound by the immobilized antibody. After incubation the wells are washed and followed by addition of HRP-conjugated Detection anti- SARS-CoV-2 (2019-nCoV) Spike RBD antibody into each well and incubated to form a complex. After washing microwells in order to remove any non-specific binding, the substrate solution (TMB) is added to microwells and color develops proportionally to the amount of SARS-CoV-2 (2019-nCoV) Spike RBD in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.
Sample Preparation and Storage
Cell Culture Supernatant: Centrifuge supernatant for 20 minutes at 1000×g at 2-8°C to remove insoluble impurity and cell debris. Collect the clear supernatant and carry out the assay immediately.
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