Mouse Anti-SARS-CoV-2 (Covid-19) to spike RBD mutation K417N, E484K, N501Y (South African Variant B.1.135) Quantitative Titration ELISA Assay Kit
The Mouse Anti-SARS-CoV-2 (Covid-19) to spike RBD mutation K417N, E484K, N501Y (South African Variant B.1.135) Quantitative Titration ELISA is For Research Use Only
Size: 12×8 wells
Standard Range: 15-1000 ng/mL
Incubation Time: 2 hours 15 minutes
Sample Type: Serum
Sample Size: 100 µL
Alternative Name: Coronavirus, SARS-CoV, Covid-19
The method employs indirect sandwich ELISA technique. SARS-CoV-2 Spike RBD (K417N, E484K, and N501Y)-His Recombinant protein is pre-coated onto microwells. Samples and standards are pipetted into microwells and Antibodies to Mouse Anti-SARS-CoV-2 (2019-nCoV) present in the sample are bound by the protein antigen. After incubation the wells are washed and followed by addition of HRP-conjugated Detection IgG Antibody into each well and incubated to form a complex. After washing microwells in order to remove any non-specific binding, the substrate solution (TMB) is added to microwells and color develops proportionally to the amount of Anti-Mouse Anti-SARS-CoV-2 (2019-nCoV) in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.
Sample Preparation and Storage
Specimens should be clear and non-hemolyzed. Samples should be run at a number of dilutions to ensure accurate quantitation. Blood is taken by venipuncture. Serum is separated after clotting by centrifugation. Plasma can be used, too. Lipaemic, hemolytic or contaminated samples should not be run. Repeated freezing and thawing should be avoided. Samples should be diluted 1:100 (v/v) for optimal recovery, (for example 1 ul sample + 99 ul (1X) Assay Diluent) prior to assay. In cases where matrix interferences is under or over observed, the samples may be diluted with Assay Diluent accordingly. The samples may be kept at 2 – 8°C for up to three days. For long-term storage please store at -20°C.
Note: Grossly hemolyzed samples are not suitable for use in this assay
Cell Culture Supernates – Remove particulates by centrifugation and assay immediately or aliquot and store samples at -20℃ or lower temperature. Avoid repeated freeze-thaw cycles. If the use of original supernate sample or low dilution (<5 fold) are necessary due to the expected low concentration of antigen supernates need be adjust to neutral pH condition before assay.
Note: The sample should be diluted to within the working range of the assay in 1X Assay Diluent. The exact dilution must be determined based on the concentration of specific target in individual samples.