SARS-CoV-2 (COVID-19) Membrane Protein Antigen Quantitative Titration ELISA Assay Kit


The Eagle Biosciences SARS-CoV-2 (Covid-19) Membrane Protein Antigen ELISA kit is used as an analytical tool for quantitative antigen determination of Human SARS-CoV-2 (Covid-19) Membrane Proteins in human serum and plasma and cell culture supernatant. Human SARS-CoV-2 (Covid-19) Membrane Protein Antigen ELISA kit is for research use only and not to be used for diagnostic procedures.

SKU: KBVH015-31 Categories: , ,

SARS-CoV-2 (COVID-19) Membrane Protein Antigen Quantitative Titration ELISA Assay Kit

The SARS-CoV-2 (COVID-19) Membrane Protein Antigen Quantitative Titration ELISA Assay Kit is For Research Use Only

Size: 12×8 wells
Specificity: <60 ng/mL
Standard Range: 62.5-4000 ng/mL
Incubation Time: 2 hours 30 minutes
Sample Type: Serum, plasma, cell culture supernatant
Sample Size: 100 µL
Alternative Name: Coronavirus, SARS-CoV, Covid-19

Assay Principle

The method employs sandwich ELISA technique. Monoclonal antibodies are pre-coated onto microwells. Samples and standards are pipetted into microwells and Human SARS-CoV-2 (Covid-19) Membrane Proteins present in the sample are bound by the capture antibodies. After incubation the wells are washed and followed by HRP-conjugated Detection Antibody is pipetted and incubated to form a complex. After washing microwells in order to remove any non-specific binding, the substrate solution (TMB) is added to microwells and color develops proportionally to the amount of Human SARS-CoV-2 (Covid-19) Envelope Proteins in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.

Sample Preparation and Storage

Specimens should be clear and non-hemolyzed. Samples should be run at a number of dilutions to ensure accurate quantitation. Blood is taken by venipuncture. Serum is separated after clotting by centrifugation. Plasma can be used, too. Lipaemic, hemolytic or contaminated samples should not be run. Repeated freezing and thawing should be avoided. Samples should be diluted 1:100 (v/v) for optimal recovery, (for example 1 ul sample + 99 ul (1X) Assay Diluent) prior to assay. In cases where matrix interferences is under or over observed, the samples may be diluted with Assay Diluent accordingly. The samples may be kept at 2 – 8°C for up to three days. For long-term storage please store at -20°C.

Note: Grossly hemolyzed samples are not suitable for use in this assay

Cell Culture Supernates – Remove particulates by centrifugation and assay immediately or aliquot and store samples at -20℃ or lower temperature. Avoid repeated freeze-thaw cycles. If the use of original supernate sample or low dilution (<5 fold) are necessary due to the expected low concentration of antigen supernates need be adjust to neutral pH condition before assay.

Note: The sample should be diluted to within the working range of the assay in 1X Assay Diluent. The exact dilution must be determined based on the concentration of specific target in individual samples.

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Additional Information

Standard Curve

SARS-CoV-2 Membrane Protein Antigen Standard Curve

Package Inserts

Please note: All documents above are for reference use only and should not be used in place of the documents included with this physical product. If digital copies are needed, please contact us.

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