SARS-CoV-2 (COVID-19) Envelope Protein Antigen Quantitative Titration ELISA Assay Kit


The Eagle Biosciences SARS-CoV-2 (Covid-19) Envelope Protein Antigen ELISA kit is used as an analytical tool for quantitative antigen determination of Human SARS-CoV-2 (Covid-19) Envelope Proteins in human serum and plasma and cell culture supernatant. The Human SARS-CoV-2 (Covid-19) Envelope Protein Antigen ELISA Assay Kit is for research use only and not to be used for diagnostic procedures.

SKU: KBVH015-30 Categories: , ,

SARS-CoV-2 (COVID-19) Envelope Protein Antigen Quantitative Titration ELISA Assay Kit

The SARS-CoV-2 (COVID-19) Envelope Protein Antigen Quantitative Titration ELISA Assay Kit is For Research Use Only

Size: 12×8 wells
Specificity: ~58 ng/mL
Standard Range: 0-5000 ng/mL
Incubation Time: 2 hours
Sample Type: Serum
Sample Size: 100 µL
Alternative Name: Coronavirus, SARS-CoV, Covid-19

Assay Principle

The method employs competitive ELISA technique. Samples and standards pipetted into microwells which are pre-coated with SARS-CoV-2 Envelope Protein antibody. SARS-CoV-2 Envelope Protein HRP:Conjugate is added which competes with the sample to form a complex. After washing microwells in order to remove any non-specific binding, the substrate solution (TMB) is added to microwells and color develops proportionally to the amount of Human SARS-CoV-2 (Covid-19) Envelope Proteins in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm. The concentration of the SARSCoV-2 Envelope Protein in the samples is inversely proportional to the yellow color developed (absorbance) in the wells.

Sample Preparation and Storage

Specimens should be clear and non-hemolyzed. Samples should be run at a number of dilutions to ensure accurate quantitation. Blood is taken by venipuncture. Serum is separated after clotting by centrifugation. Plasma can be used, too. Lipaemic, hemolytic or contaminated samples should not be run. Repeated freezing and thawing should be avoided. Samples should be diluted 1:1000 (v/v) for optimal recovery, (for example 1 ul sample + 999 ul (1X) Sample Diluent) prior to assay. In cases where matrix interferences is under or over observed, the samples may be
diluted with Sample Diluent accordingly. The samples may be kept at 2 – 8°C for up to three days. For long-term storage please store at -20°C.

Note: Grossly hemolyzed samples are not suitable for use in this assay. The sample should be diluted to within the working range of the assay in 1X Sample Diluent. The exact dilution
must be determined based on the concentration of specific target in individual samples.

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Additional Information

Standard Curve

SARS-CoV-2 Envelope Protein Antigen Standard Curve

Package Inserts

Please note: All documents above are for reference use only and should not be used in place of the documents included with this physical product. If digital copies are needed, please contact us.

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