SARS-CoV-2 (COVID-19) Envelope Protein Antigen Quantitative Titration ELISA Assay Kit
The SARS-CoV-2 (COVID-19) Envelope Protein Antigen Quantitative Titration ELISA Assay Kit is For Research Use Only
Size: 12×8 wells
Specificity: ~58 ng/mL
Standard Range: 0-5000 ng/mL
Incubation Time: 2 hours
Sample Type: Serum
Sample Size: 100 µL
Alternative Name: Coronavirus, SARS-CoV, Covid-19
The method employs competitive ELISA technique. Samples and standards pipetted into microwells which are pre-coated with SARS-CoV-2 Envelope Protein antibody. SARS-CoV-2 Envelope Protein HRP:Conjugate is added which competes with the sample to form a complex. After washing microwells in order to remove any non-specific binding, the substrate solution (TMB) is added to microwells and color develops proportionally to the amount of Human SARS-CoV-2 (Covid-19) Envelope Proteins in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm. The concentration of the SARSCoV-2 Envelope Protein in the samples is inversely proportional to the yellow color developed (absorbance) in the wells.
Sample Preparation and Storage
Specimens should be clear and non-hemolyzed. Samples should be run at a number of dilutions to ensure accurate quantitation. Blood is taken by venipuncture. Serum is separated after clotting by centrifugation. Plasma can be used, too. Lipaemic, hemolytic or contaminated samples should not be run. Repeated freezing and thawing should be avoided. Samples should be diluted 1:1000 (v/v) for optimal recovery, (for example 1 ul sample + 999 ul (1X) Sample Diluent) prior to assay. In cases where matrix interferences is under or over observed, the samples may be
diluted with Sample Diluent accordingly. The samples may be kept at 2 – 8°C for up to three days. For long-term storage please store at -20°C.
Note: Grossly hemolyzed samples are not suitable for use in this assay. The sample should be diluted to within the working range of the assay in 1X Sample Diluent. The exact dilution
must be determined based on the concentration of specific target in individual samples.
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