Mouse Flt-3ligand ELISA Kit

$515.00

The Mouse FLT-3ligand ELISA Kit is intended for the quantitation of Mouse FLT3LG concentrations in cell culture supernates and serum. The Eagle Biosciences Mouse FLT-3ligand ELISA Kit is for research use only and not for diagnostic or therapeutic procedures.

Mouse Flt-3ligand ELISA Kit

The Mouse Flt-3ligand ELISA Kit is For Research Use Only

Size: 1×96 wells
Sensitivity: <10pg/ml
Standard Range: 31.2pg/ml-2000pg/ml
Incubation Time: 2 hours 40 minutes
Sample Type: Cell culture supernates and Serum
Sample Size: 100 µl
Alternative Names: Fms-related tyrosine kinase 3 ligand, FLT3LG, or FL


Assay Principle

The Mouse FLT3LG Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Mouse FLT3LG with a 96-well strip plate that is pre-coated with antibody specific for FLT3LG. The detection antibody is a biotinylated antibody specific for FLT3LG. The capture antibody is monoclonal antibody from rat, the detection antibody is polyclonal antibody from goat. The kit contains recombinant Mouse FLT3LG with immunogen: Expression system for standard: NSO; Immunogen sequence: G27-R188. The kit is analytically validated with ready to use reagents. To measure Mouse FLT3LG, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Mouse FLT3LG in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells’ readings against the standard curve to determine the concentration of Mouse FLT3LG in the sample.


Related Products

Human Flt-3ligand ELISA Kit
Rat Flt-3 ligand ELISA Kit
Mouse FLT4/VEGFR3 ELISA Kit

Additional Information

Assay Background


Fms-related tyrosine kinase 3 ligand (FLT3LG), also known as Flt-3ligand or FL, is a protein which in humans is encoded by the FLT3LG gene. It is mapped to 19q13.3. Flt-3ligand is a hematopoietic four helical bundle cytokine. It is structurally homologous to stem cell factor (SCF) and colony stimulating factor 1(CSF-1). In synergy with other growth factors, Flt-3ligand stimulates the proliferation and differentiation of various blood cell progenitors. Besides that, Flt-3ligand can control the development of Dendritic cells (DCs) and is particularly important for plasmacytoid DCs and CD8-positive classical DCs and their CD103-positive tissue counterparts. Flt-3ligand also can enhance the response of stem and primitive progenitor cells to other growth factors to generate all myeloid lineages except erythroid cells.

Typical Standard Curve


Documents

Product Documents


 

Please note: All documents above are for reference use only and should not be used in place of the documents included with this physical product. If digital copies are needed, please contact us.

Product Citations