Human Flt-3ligand ELISA Kit


The Eagle Biosciences Human FLT-3ligand ELISA Kit is intended for the quantitation of Human FLT3LG concentrations in cell culture supernates, lysates, tissue, serum and plasma (heparin, EDTA). The Eagle Biosciences Human FLT-3ligand ELISA Kit is for research use only and not for diagnostic or therapeutic procedures.

Human Flt-3ligand ELISA Kit

The Human Flt-3ligand ELISA Kit is For Research Use Only

Size: 1×96 wells
Sensitivity: <10pg/ml
Standard Range: 15.6pg/ml-1000pg/ml
Incubation Time: 2 hours 40 minutes
Sample Type: cell culture supernates, serum and plasma(heparin, EDTA, citrate).
Sample Size: 100 µl
Alternative Names: FLT3LG, Fms-related tyrosine kinase 3 ligand, FL

Assay Principle

The Human FLT3LG Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human FLT3LG with a 96-well strip plate that is pre-coated with antibody specific for FLT3LG. The detection antibody is a biotinylated antibody specific for FLT3LG. The capture antibody is monoclonal antibody from mouse, the detection antibody is polyclonal antibody from goat. The kit contains recombinant Human FLT3LG with immunogen: Expression system for standard: sf21; Immunogen sequence: T27-P185. The kit is analytically validated with ready to use reagents. To measure Human FLT3LG, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Human FLT3LG in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells’ readings against the standard curve to determine the concentration of Human FLT3LG in the sample.

Related Products

Human FLT3 ELISA Kit
Mouse Flt-3ligand ELISA Kit
Rat Flt-3 ligand ELISA Kit

Additional Information

Assay Background

Fms-related tyrosine kinase 3 ligand(FLT3LG), also known as Flt-3ligand or FL, is a protein which in humans is encoded by the FLT3LG gene. It is mapped to 19q13.3. Flt-3ligand is a hematopoietic four helical bundle cytokine. It is structurally homologous to stem cell factor (SCF) and colony stimulating factor 1(CSF-1). In synergy with other growth factors, Flt-3ligand stimulates the proliferation and differentiation of various blood cell progenitors. Besides that, Flt-3ligand can control the development of Dendritic cells (DCs) and is particularly important for plasmacytoid DCs and CD8-positive classical DCs and their CD103-positive tissue counterparts. Flt-3ligand also can enhance the response of stem and primitive progenitor cells to other growth factors to generate all myeloid lineages except erythroid cells.

Typical Standard Curve


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