Rat Ferritin ELISA Assay

$490.00

The Rat Ferritin ELISA Assay Kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring FERRITIN in biological samples of rats.

SKU: RFE21-K01 Categories: , ,

Rat Ferritin ELISA Assay

The Rat Ferritin ELISA Assay is For Research Use Only

Size: 1×96 wells
Sensitivity: 3.014 ng/ml
Dynamic Range: 12.5 ng/ml – 400 ng/ml
Incubation Time: 80 minutes
Sample Type: Plasma, Serum
Sample Size: 100 μL


Assay Principle

The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the FERRITIN present in samples reacts with the anti-FERRITIN antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-Ferritin antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound Ferritin. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of rat Ferritin in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of rat Ferritin in the test sample. The quantity of rat Ferritin in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.


SPECIMEN COLLECTION AND HANDLING
Blood should be collected by venipuncture. The serum should be separated from the cells after clot formation by centrifugation. For plasma samples, blood should be collected into a container with an anticoagulant and then centrifuged. Care should be taken to minimize hemolysis, excessive hemolysis can impact your results. Assay immediately or aliquot and store samples at -20C. Avoid repeated freeze thaw cycles.


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Additional Information

Assay Background


Ferritin is a water-soluble, iron storage protein. Serum Ferritin level is said to be useful for diagnosis of iron deficiency anemia, metabolism disorder and malignant tumor. Ferritin may also be an acute-phase protein and is often elevated in the course of disease.

Typical Standard Curve


Rat Ferritin ELISA Assay

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