Proinsulin ELISA Assay Kit
The Proinsulin ELISA Assay Kit is For Research Use Only
Size: 1×96 wells
Standard Range: 31.2pg/mL-2000pg/mL
Incubation Time: 2 hours 35 minutes
Sample Type: Serum and plasma
Sample Size: 50 µl
The Proinsulin assay kit is a quantitative sandwich ELISA. The immunoplate is pre-coated with a monoclonal antibody specific for human proinsulin. Standards and samples are pipetted into the wells and any human proinsulin present is bound by the immobilized antibody. After washing away any unbound substances, a biotin labelled monoclonal antibody specific for human proinsulin is added to the wells. After wash step to remove any unbound reagents, streptavidin-HRP conjugate (STP-HRP) is added. After the last wash step, an HRP substrate solution is added and color develops in proportion to the amount of human proinsulin bound initially. The assay is stopped and the optical density of the wells determined using a microplate reader. Since the increases in absorbance are directly proportional to the amount of captured human proinsulin, the unknown sample concentration can be interpolated from a reference curve included in each assay kit.