Proinsulin ELISA Assay Kit


The Proinsulin ELISA Assay Kit is designed for quantification of human proinsulin in serum and plasma samples. The Eagle Biosciences Proinsulin ELISA Assay Kit is for research use only and not to be used for diagnostic purposes.

SKU: PIN31-K01 Categories: , ,

Proinsulin ELISA Assay Kit

The Proinsulin ELISA Assay Kit is For Research Use Only

Size: 1×96 wells
Sensitivity: 31.2pg/mL
Standard Range: 31.2pg/mL-2000pg/mL
Incubation Time: 2 hours 35 minutes
Sample Type: Serum and plasma
Sample Size: 50 µl

Assay Principle

The Proinsulin assay kit is a quantitative sandwich ELISA. The immunoplate is pre-coated with a monoclonal antibody specific for human proinsulin. Standards and samples are pipetted into the wells and any human proinsulin present is bound by the immobilized antibody. After washing away any unbound substances, a biotin labelled monoclonal antibody specific for human proinsulin is added to the wells. After wash step to remove any unbound reagents, streptavidin-HRP conjugate (STP-HRP) is added. After the last wash step, an HRP substrate solution is added and color develops in proportion to the amount of human proinsulin bound initially. The assay is stopped and the optical density of the wells determined using a microplate reader. Since the increases in absorbance are directly proportional to the amount of captured human proinsulin, the unknown sample concentration can be interpolated from a reference curve included in each assay kit.

Related Products

Insulin ELISA Assay Kit
Insulin-Like Growth Factor Binding Protein-1 ELISA Kit
Insulin Ultrasensitive ELISA Kit

Additional Information

Assay Background

Proinsulin is a polypeptide of 86 amino acids made in the beta cells of the pancreas and is the precursor molecule for insulin, most proinsulin is converted to insulin and C-peptide secreted into the blood. Increased circulating levels of proinsulin are found in patients with insulinomas, hypoglycemia and hyperinsulinema.


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