PMN-Elastase Stool ELISA Assay Kit
PMN-Elastase Stool ELISA Assay Kit manufactured in Germany by Immundiagnostik
Size: 1×96 wells
Dynamic Range: 0.37 – 10 ng/ml
Sensitivity: < 62 ng/ml
Incubation Time: 3 hours, 10-20 minutes
Sample Type: Stool
Sample Size: 15 mg
Alternative Names: Polymorphonuclear Neutrophil Stool ELISA, Human PMN-Elastase Stool ELISA
For Research Use Only
In a first incubation step of this ELISA, PMN elastase in the sample is bound to polyclonal rabbitanti-PMN elastase antibodies, which are immobilised on the surface of the microtiter wells. To remove all unbound substances, a washing step is carried out. In a second incubation step, a monoclonal mouse-anti-PMN elastase antibody is added. This antibody is able to detect both the free and the complexed form with the specific inhibitor (α1-proteinase inhibitor = α1-antitrypsin). The quantification of the bound PMN elastase is carried out by adding an anti-mouse peroxidase-labeled conjugate.
Finally, the PMN elastase-antigen-antibody-complex is incubated with the peroxidase substrate, tetramethylbenzidine. An acidic stop solution is then added to terminate the reaction. The color changes from blue to yellow. The intensity of the yellow color is directly proportional to the concentration of PMN elastase in the sample. A dose response curve of the absorbance unit (optical density, OD) vs. concentration is generated, using the values obtained from the standards. PMN elastase, present in the patient samples, is determined directly from this curve.
Quality Control and Reference Range
We recommend the use of external controls for internal quality control, if possible. Control samples should be analysed with each run. Results, generated from the analysis of control samples, should be evaluated for acceptability using appropriate statistical methods. The results for the patient samples may not be valid if within the same assay one or more values of the quality control sample are outside the acceptable limits.
1g stool is equivalent to 1ml
PMN elastase concentrations in faeces of healthy persons (n = 76): < 62 ng/ml
We recommend each laboratory to establish its own reference concentration range.