Alpha 1-Microglobulin ELISA Assay Kit

$540.00

The Alpha 1-Microglobulin ELISA Assay Kit is an enzyme immunoassay intended for the quantitative determination of α1-microglobulin in serum, plasma and urine. This Eagle Biosciences Alpha 1-Microglobulin ELISA is intended for research use only and should not be used in diagnostic procedures.

SKU: KR6710 Categories: , ,

Alpha 1-Microglobulin ELISA Assay Kit

Alpha 1-Microglobulin ELISA Assay Kit manufactured in Germany by Immundiagnostik

Size: 1×96 wells
Dynamic Range: 0.019-1.5 mg/l
Sensitivity: Plasma or serum: < 60 mg/l, Urine: < 12 mg/l
Incubation Time: 1 hour, 15 minutes
Sample Type: Plasma, Serum, Urine
Sample Size: 10 µl
Alternative Names: Human Alpha 1-Microglobulin ELISA, Human A1M ELISA, A1M ELISA Assay Kit
For Research Use Only
Controls Included


Assay Principle

The Alpha 1-Microglobulin ELISA Assay Kit is designed for the quantitative determination of α1-microglobulin in serum, plasma and urine. The α1-microglobulin in the samples is bound to an excess of polyclonal rabbit anti-α1-microglobulin antibodies immobilised to the surface of the microtitre plate. After a washing step to remove all foreign substances, the quantification of the bound α1-microglobulin is carried out by adding a peroxidase labelled antibody, which also binds to the α1-microglobulin. The amount of converted per- oxidase substrate is directly proportional to the amount of bound α1-microglobulin. A dose response curve of the absorbance unit (optical density, OD at 450nm) vs. concentration is generated, using the values obtained from the standard. α1- microglobulin, present in the patient samples, is determined directly from this curve.


Quality Control and Reference Range:
We recommend the use of external controls for internal quality control, if possible. Control samples should be analysed with each run. Results, generated from the ;analysis of control samples, should be evaluated for acceptability using appropriate statistical methods. The results for the patient samples may not be valid if within the same assay one or more values of the quality control sample are outside the acceptable limits.
Reference range:
Based on Immundiagnostik AG in-house studies of samples of apparently healthy persons, the following mean values were estimated.
Plasma or serum: < 60 mg/l
Urine: < 12 mg/l
We recommend each laboratory to establish its own reference range.


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Additional Information

Assay Background


α1-microglobulin, a glycoprotein heterogeneous in charge, was reported to occur both as a monomer of 31 kDa as well as a polymer of 90 kDa formed by a covalent binding with one of two alpha chains of the monomeric immunoglobulin A.
α1-microglobulin is a protein with a small molecular weight produced in the liver. In healthy persons it is metabolised in the kidneys and only minor amounts can be detected in the urine.
Increased α1-microglobulin concentration in serum is detected when the glomerular filtration rate is limited. In addition, when the ratio of total protein to the sum of albu- min and α1-microglobulin is disordered, a prerenal proteinuria should be suspected.

Manual

Product Manual