Alpha 1-Microglobulin ELISA Assay Kit

$520.00

The Eagle Biosciences Alpha 1-Microglobulin ELISA Assay Kit is an enzyme immunoassay intended for the quantitative determination of α1-microglobulin in serum, plasma and urine. This immunoassay is intended for research use only and should not be used in diagnostic procedures.

SKU: KR6710 Categories: , ,

Alpha 1-Microglobulin ELISA Assay Kit

For Research Use Only

Size: 1×96 wells
Dynamic Range: 0.019-1.5 mg/l
Sensitivity: Plasma or serum: < 60 mg/l, Urine: < 12 mg/l
Incubation Time: 1 hour, 15 minutes
Sample Type: Plasma, Serum, Urine
Sample Size: 10 µl

Product manufactured in Germany by Immundiagnostik

Additional Information

Assay Background

α1-microglobulin, a glycoprotein heterogeneous in charge, was reported to occur both as a monomer of 31 kDa as well as a polymer of 90 kDa formed by a covalent binding with one of two alpha chains of the monomeric immunoglobulin A.
α1-microglobulin is a protein with a small molecular weight produced in the liver. In healthy persons it is metabolised in the kidneys and only minor amounts can be detected in the urine.
Increased α1-microglobulin concentration in serum is detected when the glomerular filtration rate is limited. In addition, when the ratio of total protein to the sum of albu- min and α1-microglobulin is disordered, a prerenal proteinuria should be suspected.

Assay Principle

The Eagle Biosciences Alpha 1-Microglobulin ELISA Assay Kit is designed for the quantitative determination of α1-microglobulin in serum, plasma and urine. The α1-microglobulin in the samples is bound to an excess of polyclonal rabbit anti-α1-microglobulin antibodies immobilised to the surface of the microtitre plate. After a washing step to remove all foreign substances, the quantification of the bound α1-microglobulin is carried out by adding a peroxidase labelled antibody, which also binds to the α1-microglobulin. The amount of converted per- oxidase substrate is directly proportional to the amount of bound α1-microglobulin. A dose response curve of the absorbance unit (optical density, OD at 450nm) vs. concentration is generated, using the values obtained from the standard. α1- microglobulin, present in the patient samples, is determined directly from this curve.

Manual

Product Manual