H. pylori Qualitative ELISA
H. pylori Qualitative ELISA Developed and Manufactured in the USA
Size: 1×96 wells
Sensitivity: Cut-off Control
Incubation Time: 2 hours
Sample Type: Stool
Sample Size: 40 mg
Alternative Names: Helicobacter pylori Antigen, H. pylori ELISA
For Research Use Only
The Helicobacter pylori ELISA is a “sandwich” ELISA designed, developed and produced for the qualitative measurement of H. pylori antigen in stool specimen. The assay utilizes the microplate-based enzyme immunoassay technique by coating highly purified antibody onto the wall of microtiter wells.
Assay calibrators and fecal specimen are added to microtiter wells of microplate that was coated with a highly purified monoclonal H. pylori antibody on its wall. During the assay, the H. pylori antigen will be bound to the antibody coated plate after an incubation period. The unbound material is washed away and another HRP-conjugated monoclonal antibody which specifically recognizes the protein of H. pylori is added for further immunoreactions. After an incubation period, the immunocomplex of “H. pylori Antibody – H. pylori Antigen – HRP-conjugated Anti-H. pylori Tracer Antibody” is formed if H. pylori antigen is present in the test sample. The unbound tracer antibody and other proteins in buffer matrix are removed in the subsequent washing step. HRP conjugated tracer antibody bound to the well is then incubated with a substrate solution in a timed reaction and then measured in a spectrophotometric microplate reader. The enzymatic activity of the tracer antibody bound to H. pylori proteins captured on the wall of each microtiter well is directly proportional to the amount of H. pylori antigen level in each test specimen.
The H. pylori Ag ELISA does not cross react to the following organisms: Cryptosoridium parvum, Giardia lamblia, rotavirus and adenovirus.