Beta 2 Microglobulin ELISA Assay Kit
Beta 2 Microglobulin ELISA Assay Kit manufactured in Germany by Immundiagnostik
Size: 1×96 wells
Dynamic Range: 0.6-10 mg/l
Sensitivity: Plasma or serum: < 2.5 mg/l, Urine: < 0.4 mg/l
Incubation Time: 3.5 hours
Sample Type: Plasma, Serum, Urine
Sample Size: 10 µl
Alternative Names: Human Beta 2-Microglobulin ELISA, B2M ELISA, ß2-M ELISA
For Research Use Only
This Beta 2 Microglobulin ELISA Assay Kit is designed for the quantitative determination of ß2-microglobulin. In a first inicubation step, ß2-microglobulin is bound to an immobilised antibody.Then a peroxidase-labelled anti-ß2-microglobulin-antibody is added and a sandwich of capture antibody – ß2-microglobulin – peroxidase-conjugate is formed. Tetramethylbenzidine (TMB) is used as peroxidase substrate. Finally, an acidic stop solution is added to terminate the reaction. The colour changes from blue to yellow. The intensitiy of the colour is directly proportional to the concentration of ß2-microglobulin. A dose response curve of the absorbance unit (optical density, OD at 450nm) vs. concentration is generated, using the values obtained from the standards. ß2-microglobulin, present in the patient samples, is determined directly from this curve.
Serum and Plasma:
Samples can be stored for two weeks at 2-8 °C. For longer storage, samples should be frozen at -20 °C.
Urines should be adjusted to a pH of 6 to 8 with 1 N NaOH. Adjusted samples can be stored at 2-8 °C for 14 days. For longer storage, non-treated samples should be frozen; at -20 °C.
Dilution of samples:
Plasma, serum and urine samples must be diluted 1:50 before performing the assay, ;mix well. For example 10µl sample + 490µl sample dilution buffer (SAMPLEBUF). Samples with a ß2 -microglobulin content higher than 10 mg/l should be further diluted 1:10 with sample dilution buffer. For analysis, pipet 10µl of the dilution per well
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