Beta 2 Microglobulin ELISA Assay Kit


This Beta-2 Microglobulin ELISA Kit is an enzyme immunoassay intended for the quantitative determination of Beta 2-microglobulin in serum, plasma and urine. Beta 2- Microglobulin Assay is for researach use only and should not be used for diagnostic procedures.

SKU: KR6210 Categories: ,

Beta 2 Microglobulin ELISA Assay Kit

Beta 2 Microglobulin ELISA Assay Kit manufactured in Germany by Immundiagnostik

Size: 1×96 wells
Dynamic Range: 0.6-10 mg/l
Sensitivity: Plasma or serum: < 2.5 mg/l, Urine: < 0.4 mg/l
Incubation Time: 3.5 hours
Sample Type: Plasma, Serum, Urine
Sample Size: 10 µl
Alternative Names: Human Beta 2-Microglobulin ELISA, B2M ELISA, ß2-M ELISA
For Research Use Only
Controls Included

Assay Principle

This Beta 2 Microglobulin ELISA Assay Kit is designed for the quantitative determination of ß2-microglobulin. In a first inicubation step, ß2-microglobulin is bound to an immobilised antibody.Then a peroxidase-labelled anti-ß2-microglobulin-antibody is added and a sandwich of capture antibody – ß2-microglobulin – peroxidase-conjugate is formed. Tetramethylbenzidine (TMB) is used as peroxidase substrate. Finally, an acidic stop solution is added to terminate the reaction. The colour changes from blue to yellow. The intensitiy of the colour is directly proportional to the concentration of ß2-microglobulin. A dose response curve of the absorbance unit (optical density, OD at 450nm) vs. concentration is generated, using the values obtained from the standards. ß2-microglobulin, present in the patient samples, is determined directly from this curve.

Sample storage:
Serum and Plasma:
Samples can be stored for two weeks at 2-8 °C. For longer storage, samples should be frozen at -20 °C.
Urines should be adjusted to a pH of 6 to 8 with 1 N NaOH. Adjusted samples can be stored at 2-8 °C for 14 days. For longer storage, non-treated samples should be frozen; at -20 °C.
Dilution of samples:
Plasma, serum and urine samples must be diluted 1:50 before performing the assay, ;mix well. For example 10µl sample + 490µl sample dilution buffer (SAMPLEBUF). Samples with a ß2 -microglobulin content higher than 10 mg/l should be further diluted 1:10 with sample dilution buffer. For analysis, pipet 10µl of the dilution per well

Related Products

Alpha 1-Microglobulin ELISA
alpha 2-Macroglobulin (a2-M) ELISA Assay
Rat Beta 2-Microglobulin ELISA

Additional Information

Assay Background

ß2-microglobulin is a light chain protein (11.8kD) of the HLA-class-I antigens and is found on the cell membrane of all nucleated cells. This protein is metabolised extensively in the kidney. The serum concentration is influenced by the rates of synthesis and metabolism and is usually stable in healthy persons. Changes in the serum concentrations are indicative of disorders in glomerular and tubular functions.

Package Inserts

Please note: All documents above are for reference use only and should not be used in place of the documents included with this physical product. If digital copies are needed, please contact us.

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