Niacin Microtiter Plate Assay Kit
Niacin Microtiter Plate Assay Kit manufactured in Germany by Immundiagnostik
Size: 1×96 wells
Sensitivity: 2 µg/L
Dynamic Range: 2 – 40 µg/L
Incubation Time: 48 hours
Sample Type: Serum
Sample Size: 100 µL
For Research Use Only
Controls Included
Assay Principle
In the Niacin Microtiter Plate Assay Kit, serum samples are diluted and added into the microtiter plate wells coated with Lactobacillus plantarum which metabolizes niacin. The presence of niacin both in standards [STD] and samples gives a niacin-dependent growth response. After incubation at 37°C for 48 hours, the growth of Lactobacillus plantarum is measured turbidimetrically at 610 – 630 nm (alternative at 540 – 550 nm) in an ELISA-reader. A dose-response curve of absorbance unit (optical density, OD at 610 nm) vs. concentration is generated using the values obtained from the standard. Niacin present in the patient samples is determined directly from this curve.
Niacin deficiency syndromes:
Symptoms of minor niacin deficiency:
– Loss of appetite
– Depression
– Dementia
– Insomnia
– Weakness
– Irritability
Severe niacin deficiency may cause pellagra. The term pellagra is derived from the Italian words“pelle agra” meaning “rough” or “smarting skin”. Pellagra is characterized by symptoms such as:
– Glossitis
– Sore, swollen, purple-red tongue
– Skin lesions primarily located on sun-exposed areas
Niacin as a cholesterol lowering drug:
Niacin increases HDL cholesterol and reduces LDL cholesterol and triglycerides. When taken in conjunction with another cholesterol medication, diet or exercise, niacin has been proven to reduce “bad” cholesterol levels. A niacin-stat in combination therapy substantially improves 4 major lipoprotein levels associated with atherosclerotic disease (Insull et al. 2004). The drug combination had good records in clinical trials for reduction in cardiovascular events and improvement in progression/regression of coronary lesions.
Indications:
– Deeply pigmented skin on sun-exposed areas
– Alcohol abuse
– Dementia
– Dry skin and mouth
– Numbness of the extremities
– Inflammation of the mucous membranes of the tongue and mouth
– Digestive disorders
Niacin can be synthesized in the body from tryptophan,whereby the conversion requires the presence of thiamine, pyridoxine, and riboflavin. Any deficiency in these vitamins can affect the niacin metabolism.
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