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Mouse TGF-beta 3 ELISA Kit

$585.00

The Mouse TGF-beta 3 ELISA Kit is intended for the quantitation of Mouse TGFB3 concentrations in cell culture supernates, serum, plasma (EDTA) and urine. The Eagle Biosciences Mouse TGF-beta 3 ELISA Kit is for research use only and not for diagnostic or therapeutic procedures.

SKU: TB311-K01 Categories: , ,

Mouse TGF-beta 3 ELISA Kit

The Mouse TGF-beta 3 ELISA Kit is For Research Use Only

Size: 1×96 wells
Sensitivity: <10pg/ml
Standard Range: 31.2pg/ml-2000pg/ml
Incubation Time: 2 hours 40 minutes
Sample Type: Cell culture supernates, serum, plasma(EDTA) and urine
Sample Size: 100 µl
Alternative Name: Transforming growth factor beta 3, TGFB3

Assay Principle

The Mouse TGFB3 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Mouse TGFB3 with a 96-well strip plate that is pre-coated with antibody specific for TGFB3. The detection antibody is a biotinylated antibody specific for TGFB3. The capture antibody is monoclonal antibody from rat, the detection antibody is polyclonal antibody from goat. The kit contains recombinant Mouse TGFB3 with immunogen: Expression system for standard: sf21; Immunogen sequence: A301-S412. The kit is analytically validated with ready to use reagents. To measure Mouse TGFB3, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Mouse TGFB3 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells’ readings against the standard curve to determine the concentration of Mouse TGFB3 in the sample.

Related Products

Mouse TGF beta1 ELISA Assay Kit
Mouse TGF-beta 2 ELISA Kit
Mouse Thrombopoietin ELISA Kit

Additional Information

Assay Background

Transforming growth factor beta 3(TGF-beta 3) is a type of protein, known as a cytokine, which is involved in cell differentiation, embryogenesis and development. It belongs to a large family of cytokines called the Transforming growth factor beta superfamily. TGF-beta 3 is believed to regulate molecules involved in cellular adhesion and extracellular matrix(ECM) formation during the process of palate development. Without TGF beta 3, mammals develop a deformity known as a cleft palate. This is caused by failure of epithelial cells in both sides of the developing palate to fuse. TGF-beta 3 also plays an essential role in controlling the development of lungs in mammals, by also regulating cell adhesion and ECM formation in this tissue, and controls wound healing by regulating the movements of epidermal and dermal cells in injured skin. TGF-beta 3 activated Lef1 in the absence of beta-catenin(CTNNB1) via nuclear phospho-Smad2 and Smad4.

Typical Standard Curve

Mouse TGF-beta 3 ELISA Kit

Documents

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