Galectin-3 ELISA Assay Kit

$540.00

The Galectin-3 ELISA kit is designed for quantification of human galectin-3 in serum and plasma samples. The Eagle Biosciences Galectin-3 ELISA kit is for research use only and not to be used for diagnostic procedures.

Galectin-3 ELISA Assay Kit

The Galectin-3 ELISA Assay Kit is For Research Use Only

Size: 1×96 wells
Sensitivity: 0.145ng/ml
Standard Range: 0.625ng/ml – 40ng/ml
Incubation Time: 3 hrs 15 minutes
Sample Type: Serum and plasma
Sample Size: 100 µl


Assay Principle

The Galectin-3 assay kit is a quantitative sandwich ELISA. The immunoplate is pre-coated with a polyclonal antibody specific for human galectin-3. Standards and samples are pipetted into the wells and any human galectin-3 present is bound by the immobilized antibody. After washing away any unbound substances, a horseradish peroxidase (HRP)-linked polyclonal antibody specific for human galectin-3 is added to the wells. After a final wash step, an HRP substrate solution is added and color develops in proportion to the amount of human galectin-3 bound initially. The assay is stopped and the optical density of the wells determined using a microplate reader. Since the increases in absorbance are directly proportional to the amount of captured human galectin-3, the unknown sample concentration can be interpolated from a reference curve included in each assay kit.

Sample Preparation

Serum or plasma sample is generally required a 5-fold dilution in the 1×Assay


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Additional Information

Assay Background


Galectins are a family of animal lectins with carbohydrate-binding activity and specificity for N-acetyllactosamine (LacNac). Galectin-3 was the unique chimera type lectins. Galectin-3 lacks a signal peptide for classical secretion but presents and active inside and outside the cell. Human galectin-3, 29-36 KDa, exhibits numerous autocrine and paracrine effects and mediates in cell adhesion, cell activation and apoptosis and up- or down-regulation in cancer. In addition, plays an important role in immune response and inflammation. While the cytosolic galectin-3 involves in the cell proliferation, differentiation and survival.
Serum levels of galectin-3 elevated in Behcet’s Diseases, Thyroid disease, Alzheimer’s disease, cardiovascular disease such as LAA stroke and in serval cancers especially when they are metastatic. Moreover, circulating levels of galectin-3 are higher in obese humans and indicative for a role in insulin resistance in man.

Typical Standard Curve


Documents

Product Manual