Galectin-3 ELISA Assay Kit
The Galectin-3 ELISA Assay Kit is For Research Use Only
Size: 1×96 wells
Sensitivity: 0.145ng/ml
Standard Range: 0.625ng/ml – 40ng/ml
Incubation Time: 3 hrs 15 minutes
Sample Type: Serum and plasma
Sample Size: 100 µl
Assay Principle
The Galectin-3 assay kit is a quantitative sandwich ELISA. The immunoplate is pre-coated with a polyclonal antibody specific for human galectin-3. Standards and samples are pipetted into the wells and any human galectin-3 present is bound by the immobilized antibody. After washing away any unbound substances, a horseradish peroxidase (HRP)-linked polyclonal antibody specific for human galectin-3 is added to the wells. After a final wash step, an HRP substrate solution is added and color develops in proportion to the amount of human galectin-3 bound initially. The assay is stopped and the optical density of the wells determined using a microplate reader. Since the increases in absorbance are directly proportional to the amount of captured human galectin-3, the unknown sample concentration can be interpolated from a reference curve included in each assay kit.
Sample Preparation
Serum or plasma sample is generally required a 5-fold dilution in the 1×Assay
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