Mouse Fibronectin ELISA Kit
The Mouse Fibronectin ELISA Kit is For Research Use Only
Size: 1×96 wells
Standard Range: 156pg/ml-10000pg/ml
Incubation Time: 2 hours 40 minutes
Sample Type: Cell Culture Supernates, Serum, Plasma
Sample Size: 100 µl
The Mouse Fibronectin ELISA Assay Kit is a solid phase immunoassay specially designed to measure Mouse FN1 with a 96-well strip plate that is pre-coated with antibody specific for FN1. The detection antibody is a biotinylated antibody specific for FN1. The capture antibody is monoclonal antibody from rat, the detection antibody is polyclonal antibody from goat. The kit contains recombinant Mouse FN1 with immunogen: Expression system for standard: from plasma. The kit is analytically validated with ready to use reagents.
To measure Mouse FN1, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Mouse FN1 in the sample. Read the density of the yellow product in each well using a plate reader and benchmark the sample wells’ readings against the standard curve to determine the concentration of Mouse FN1 in the sample.