Mouse Fibrinogen ELISA Assay


The Mouse Fibrinogen ELISA Assay Kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Fibrinogen in biological samples of mice.

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Mouse Fibrinogen ELISA Assay

The Mouse Fibrinogen ELISA Assay is For Research Use Only

Size: 1×96 wells
Sensitivity: 2.568 ng/ml
Dynamic Range: 25 ng/ml – 800 ng/ml
Incubation Time: 100 minutes
Sample Type: Plasma, Serum
Sample Size: 100 μL

Assay Prinicple

The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the Fibrinogen present in samples reacts with the anti-Fibrinogen antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-FIB antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound FIB . Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of FIB in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of FIB in the test sample. The quantity of FIB in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

Blood should be collected by venipuncture. The serum should be separated from the cells after clot formation by centrifugation. For plasma samples, blood should be collected into a container with an anticoagulant and then centrifuged. Care should be taken to minimize hemolysis, excessive hemolysis can impact your results. Assay immediately or aliquot and store samples at -20°C. Avoid repeated freeze thaw cycles.

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Additional Information

Assay Background

Soluble Fibrinogen (FIB) circulates in the blood and provides the material from which the insoluble fibrin clot is formed during blood coagulation. Fibrinogen is an acute phase reactant that may be a useful marker for infection and inflammation. This ELISA kit can be used to measure Fibrinogen in biological samples of mice.

Typical Standard Curve

Mouse Fibrinogen ELISA Assay


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