Human Fibrinogen ELISA Assay

$380.00

The Human Fibrinogen ELISA Assay Kit is a highly sensitive two-site enzyme linked immunoassay for measuring Fibrinogen in biological samples of humans.

SKU: HFG31-K01 Categories: , ,

Human Fibrinogen ELISA Assay

The Human Fibrinogen ELISA Assay is For Research Use Only

Size: 1×96 wells
Sensitivity: 2.088 ng/ml
Dynamic Range: 12.5 ng/ml – 400 ng/ml
Incubation Time: 100 minutes
Sample Type: Plasma, Serum
Sample Size: 100 μL


Assay Principle

In this Human Fibrinogen ELISA Assay the Fibrinogen present in samples reacts with the anti-Fibrinogen antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-FIB antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound FIB. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of FIB in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of FIB in the test sample. The quantity of FIB in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.


Specimen Collection and Handling
Blood should be collected by venipuncture. The serum should be separated from the cells after clot formation by centrifugation. For plasma samples, blood should be collected into a container with an anticoagulant and then centrifuged. Care should be taken to minimize hemolysis, excessive hemolysis can impact your results. Assay immediately or aliquot and store samples at -20°C. Avoid repeated freeze thaw cycles.


Related Products

Human Hemopexin ELISA Assay
Human Hemoglobin ELISA Assay Kit
Human Haptoglobin ELISA

Additional Information

Assay Background


Soluble Fibrinogen (FIB) circulates in the blood and provides the material from which the insoluble fibrin clot is formed during blood coagulation. Fibrinogen is an acute phase reactant that may be a useful marker for infection and inflammation. This ELISA kit can be used to measure Fibrinogen in biological samples.

Typical Standard Curve


Manual

Product Manual