Mouse Adiponectin ELISA Assay
The Mouse Adiponectin ELISA Assay is For Research Use Only
Size: 1×96 wells
Sensitivity: 0.008 ng/ml
Standard Range: 0.025 – 1 ng/ml
Incubation Time: 2.5 hours
Sample Type: Serum, Plasma
Sample Size: 10 µL
Manufactured by Mediagnost
Serum and plasma samples of mice and mouse cell culture medium can be used in this assay. Influence of Heparin (30 IE/mL), EDTA (6,8 mM) and NaCitrat (0,015 M) on the measurement of Adiponectin has been investigated be recovery experiments. PBS was enriched with recombinant mouse Adiponectin and the above mentioned substances. No significant influence on the recovery of adiponectin was detected.
Hemolytic reactions have to be avoided. The blood has to be allowed to clot and after complete clotting, serum is separated by centrifugation.
The Eagle Bio Mouse Adiponectin ELISA standards are prepared from native Adiponectin in concentrations of 0.025, 0.075, 0.15, 0.3, 0.65 and 1 ng/ml and the native Adiponectin was calibrated against the recombinant Protein (Manufacturer: R&D Systems, Wiesbaden).
The Mouse Adiponectin ELISA Assay Kit is a so-called Sandwich-Assay using two specific and high affinity antibodies. The Adiponectin in the samples binds to the first antibody coated on the microtiter plate. In the following step the second specific anti-Adiponectin-Antibody binds in turn to the immobilised Adiponectin. The second antibody is biotinylated and will be applied in a mixture with a Streptavidin-Peroxidase-Enzyme Conjugate. In the closing substrate reaction the turn of the colour will be catalysed quantitatively depending on the Adiponectin-level of the samples.
The analytical sensitivity of the Mouse Adiponectin ELISA yields 0.008 ng/mL (equal to < 0.0008 ng per well; as 2xSD of zero standard in 22fold determination).