Human Adiponectin ELISA Assay

$500.00

The Human Adiponectin ELISA Assay Kit measures human Adiponectin in human serum and plasma samples. The Human Adiponectin ELISA Assay kit is for research use only.

Human Adiponectin ELISA Assay

The Human Adiponectin ELISA Assay is For Research Use Only

Size: 1×96 wells
Sensitivity: <0.27 ng/ml Standard Range: 2-100 ng/mL
Incubation Time: 1.75 hours
Sample Type: Serum, Plasma
Sample Size: 10 µL

Manufactured by Mediagnost


Assay Background

Adiponectin is a 30kDa protein which percentage in serum proteins is 0.01%. It is mainly synthesized by Adipocytes, but also muscle cells and hepatocytes have the ability to synthesize Adiponectin. Until now, IGF-I is the only known natural inductor of the synthesis. It consists of  a Collagen-like N-terminal and a globular C-terminal domain. In vivo Adiponectin appears with different oligomers. Beside the trimer and ditrimer also high molecular multimers exist. Up to now two different receptors are known, both receptors are ubiquitary expressed, though the distribution in the tissues varies. The Adiponectin Receptor 1 (AdipoR1) is especially in muscle- and AdipoR2 in liver tissue synthesized.

The significance for the human organism is not clear until now. First studies show, that adiponectin correlates negatively with BMI and thus it could have relevance for the energy metabolism for example through the regulation of fatty acid oxidation. Beside the correlation with BMI, Adiponectin level is associated with the Insulin-Resistance and so also linked with Type II Diabetes. Adiponectin is associated also with glucose- und lipometabolim.

Furthermore it is involved in inflammatory processes and therewith it is of importance for appearance of arteriosclerosis and coronaritis, thus the determination of Adiponectin level  in plasma could serve to estimate the risk of coronary disease. Beside this Adiponectin influences further physiological processes as for example the angiogenesis.


Specimen
Serum and heparin plasma levels are comparable. In EDTA- and Citrate Plasma-samples levels were found approx. 18% lower, because of the relatively high amount of anticoagulant. Adiponectin can also be measured in urine, breast milk and cell culture media by this test system. The blood sample for serum preparation should be gained according to standardized venipucture procedure. The samples should be stored without anticoagulation reagents. Hemolytic reactions have to be avoided. The blood has to be allowed to clot and after complete clotting, serum is separated by centrifugation.


Related Products

Mouse Adiponectin ELISA Kit
Rat Adiponectin ELISA Assay
Adiponectin High Sensitive ELISA Assay Kit

Additional Information

Assay Principle


The Eagle Biosciences Adiponectin ELISA Assay Kit is a so-called Sandwich-Assay using two specific and high affinity antibodies. The Adiponectin in the samples binds to the first antibody coated on the microtiter plate. In the following step the second specific anti-Adiponectin-Antibody binds in turn to the immobilised Adiponectin. The second antibody is biotinylated and will be applied in a mixture with a Streptavidin-Peroxidase-Enzyme Conjugate. In the closing substrate reaction the turn of the colour will be catalysed quantitatively depending on the Adiponectin-level of the samples.

Standards


The Standards of the Adiponectin ELISA Assay are prepared from native Adiponectin (Human Serum) in concentrations of 2, 10, 30, 70 and 100 ng/ml. The native Adiponectin was quantified with a recombinant protein and with a commercialised radio immunoassay for Adiponectin.

Human Adiponectin ELISA Assay

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