Monoclonal Mouse Anti-Human HER2/neu Antibody
The Monoclonal Mouse Anti-Human HER2/neu Antibody is For Research Use Only
Clone: IHC042
Source: Mouse Monoclonal
Positive Control: Breast Carcinoma
Localization: Membranous
Background
The HER2/neu (c-erbB-2) proto-oncogene is a transmembrane receptor tyrosine kinase that is clinically indicated in a number of carcinomas. Overexpression of the c-erbB-2 protein has been associated with ductal breast cancer, as well as pulmonary and gastric adenocarcinomas. A correlation between HER2 and p53 has also been documented, as overexpression of both proteins has been associated with early invasion and metastasis in bladder cancer.
Antibody Principle
Visualization of the antigen present in tissue sections is accomplished in a multistep
immunohistochemical staining process, in conjunction with a horseradish
peroxidase (HRP) or alkaline phosphatase (AP) linked detection system. The process
involves the addition of the stated antibody (primary antibody) to a tissue slide,
followed by a secondary antibody (linked to an enzyme complex) which specifically
binds to the primary antibody. A chromogenic substrate is then added which reacts
with the enzyme complex, resulting in a colorimetric reaction at the site of the
antigen. Results are interpreted using a light microscope.
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