Monoclonal Mouse Anti-Human IDH1 R132H Antibody

$160.00$570.00

The Eagle Biosciences Monoclonal Mouse Anti-Human IDH1 R132H antibody is intended for qualified laboratories to qualitatively identify by light microscopy, the presence of associated antigens in formalin-fixed, paraffin-embedded (FFPE) tissue sections using immunohistochemistry test methods. The Monoclonal Mouse Anti-Human ODH1 R132H Antibody is for research use only and not to be used for diagnostic procedures.

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SKU: IHC132 Categories: ,

Monoclonal Mouse Anti-Human IDH1 R132H Antibody

The Monoclonal Mouse Anti-Human IDH1 R132H Antibody is For Research Use Only

Clone: IHC132
Source: Mouse Monoclonal
Positive Control: Glioma
Localization: Cytoplasmic


Background

Isocitrate Dehydrogenase 1 (IDH1) is a soluble, cytoclic enzyme involved in the TCA metablic cycle. The most notable mutation in this enzyme, R132H, is clinically indicated in the majority of astrocytomas and oligodendroglial tumours, with the mutation being associated with more favourable prognosis and increased survival in those patients. IDH1 R132H is also useful in the differential diagnosis between anaplastic glioma and glioblastoma.

Antibody Principle

Visualization of the antigen present in tissue sections is accomplished in a multi-step immunohistochemical staining process, in conjunction with a horseradish peroxidase (HRP) or alkaline phosphatase (AP) linked detection system. The process involves the addition of the stated antibody (primary antibody) to a tissue slide, followed by a secondary antibody (linked to an enzyme complex) which specifically binds to the primary antibody. A chromogenic substrate is then added which reacts with the enzyme complex, resulting in a colorimetric reaction at the site of the antigen. Results are interpreted using a light microscope.


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