Lysozyme (Stool) ELISA Assay Kit
Lysozyme Stool ELISA Assay Kit manufactured in Germany by Immundiagnostik
Size: 1×96 wells
Standard Range: 1.1-30 ng/ml
Incubation Time: 1h; 1h; 10-20min
Sample Type: Stool
Sample Size: 15 mg
For Research Use Only
Assay Principle
The Lysozyme Stool ELISA Assay Kit assay utilizes the “sandwich” technique with two selected antibodies that recognise human lysozyme.
Standards, controls and diluted samples, which are assayed for human lysozyme, are added into the wells of a micro plate coated with a high affine anti-human lysozyme antibody. During the first incubation step, lysozyme is bound by the immobilised antibody. Then a peroxidase-conjugated anti-human lysozyme antibody is added into each microtiter well and a “sandwich” of capture antibody – human lysozyme – peroxidase-conjugate is formed. Tetramethylbenzidine is used as peroxidase sub-strate. Finally, an acidic stop solution is added to terminate the enzymatic reaction. The colour changes from blue to yellow. The intensity of the yellow colour is directly proportional to the concentration of lysozyme. A dose response curve of the absorbance unit (optical density, OD at 450 nm) vs. concentration is generated using the values obtained from the standards. Lysozyme present in the samples is determined directly from this curve.
Indications
• Diagnosis and monitoring of Crohn’s Disease
• Early diagnosis of rejection reactions in kidney transplantation cases
• Differential diagnosis and monitoring of leukosis
• Diagnosis and treatment monitoring of urinary tract infections in children
• Differential diagnosis between viral and bacterial meningitis in children
• Identification of sepsis in neonates
Quality Control and Reference Range
We recommend the use of external controls for internal quality control, if possible.
Control samples should be analysed with each run. Results, generated from the analysis of control samples, should be evaluated for acceptability using appropriate statistical methods. The results for the patient samples may not be valid if within the same assay one or more values of the quality control sample are outside the acceptable limits.
Reference range
Based on studies of stool samples of apparently healthy persons (n = 80), a reference value of lysozyme < 600ng/ml was estimated. We recommend each laboratory to establish its own reference range.
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