Human CD62L ELISA Assay
The Human CD62L ELISA Assay is For Research Use Only
Size: 1×96 wells
Sensitivity: 1 ng/ml
Dynamic Range: 1.6 ng/ml – 60 ng/ml
Incubation Time: 1h 45 min
Sample Type: Serum, Plasma, Cell Culture
Sample Size: 100 µl
Alternative Names: LAM-1, L-Selectin
Specimen collection, processing & storage
Cell culture supernatants, serum, plasma or other biological samples will be suitable for use in the assay. Remove serum from the clot or red cells, respectively, as soon as possible after clotting and separation.
Cell culture supernatants:
Remove particulates and aggregates by spinning at approximately 1000 x g for 10 min.
Avoid any unintentional stimulation of the cells by the procedure. Use pyrogen/endotoxin free collecting tubes. Serum should be removed rapidly and carefully from the red cells after clothing. For that, after clothing, centrifuge at approximately 1000 x g for 10 min and remove serum.
EDTA, citrate and heparin plasma can be assayed. Spin samples at 1000 x g for 30 min to remove particulates. Harvest plasma.
If not analyzed shortly after collection, samples should be aliquoted (250-500μl) to avoid repeated freeze-thaw cycles and stored frozen at –70°C. Avoid multiple freeze-thaw cycles of frozen specimens.
L-selectin, also known as CD62L, is a cell adhesion molecule found on leukocytes and preimplantation embryos. It belongs to the family of selectin proteins, which recognize sialylated carbohydrate groups. It is cleaved by ADAM17.
SELL is a cell surface component that belongs to a family of adhesion / referral receptors that play an important role in lymphocyte-endothelial cell interactions. The molecule is composed of several domains: one homologue for lectins, one for epidermal growth factor and two for consensus repeat units found in C3 / C4 binding proteins.