Human CD62L ELISA Assay

$560.00

The Human CD62L ELISA Assay is to be used for the in-vitro quantitative determination CD62L in human serum, plasma, buffered solutions or cell culture medium. The assay will recognize both natural and recombinant human CD62L. The Eagle Biosciences Human CD62L ELISA Assay kit is for Research Use Only.

SKU: C2L31-K01 Categories: , ,

Human CD62L ELISA Assay

The Human CD62L ELISA Assay is For Research Use Only

Size: 1×96 wells
Sensitivity: 1 ng/ml
Dynamic Range: 1.6 ng/ml – 60 ng/ml
Incubation Time: 1h 45 min
Sample Type: Serum, Plasma, Cell Culture
Sample Size: 100 µl
Alternative Names: LAM-1, L-Selectin


Specimen collection, processing & storage
Cell culture supernatants, serum, plasma or other biological samples will be suitable for use in the assay. Remove serum from the clot or red cells, respectively, as soon as possible after clotting and separation.
Cell culture supernatants:
Remove particulates and aggregates by spinning at approximately 1000 x g for 10 min.
Serum:
Avoid any unintentional stimulation of the cells by the procedure. Use pyrogen/endotoxin free collecting tubes. Serum should be removed rapidly and carefully from the red cells after clothing. For that, after clothing, centrifuge at approximately 1000 x g for 10 min and remove serum.
Plasma:
EDTA, citrate and heparin plasma can be assayed. Spin samples at 1000 x g for 30 min to remove particulates. Harvest plasma.
Storage:
If not analyzed shortly after collection, samples should be aliquoted (250-500μl) to avoid repeated freeze-thaw cycles and stored frozen at –70°C. Avoid multiple freeze-thaw cycles of frozen specimens.


Assay Background

L-selectin, also known as CD62L, is a cell adhesion molecule found on leukocytes and preimplantation embryos. It belongs to the family of selectin proteins, which recognize sialylated carbohydrate groups. It is cleaved by ADAM17.

SELL is a cell surface component that belongs to a family of adhesion / referral receptors that play an important role in lymphocyte-endothelial cell interactions. The molecule is composed of several domains: one homologue for lectins, one for epidermal growth factor and two for consensus repeat units found in C3 / C4 binding proteins.


Related Products

Human CD62P ELISA Assay Kit
Human CD62E ELISA Assay
Human CD21 ELISA Assay Kit

Additional Information

Assay Principle


The CD62L Kit is a solid phase sandwich Enzyme Linked-Immuno- Sorbent Assay (ELISA). A monoclonal antibody specific for CD62L has been coated onto the wells of the microtiter strips provided. Samples, including standards of known CD62L concentrations and unknowns are pipetted into these wells.  During the first incubation, the CD62L antigen and a biotinylated monoclonal antibody specific for CD62L are simultaneously incubated.  After washing, the enzyme (streptavidin-peroxydase) is added. After incubation and washing to remove all the unbound enzyme, a substrate solution is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of CD62L present in the samples.

  1. Add 100µl of each standard, sample, control and zero (Standard Diluent) in duplicate to appropriate number of wells
  2. Add 50µl of diluted biotinylated anti-CD62L to all wells
  3. Cover with a plastic plate cover and incubate at room temperature (18 to 25°C) for 1 hours
  4. Remove the cover and wash the plate
  5. Add 100μl of Streptavidin-HRP solution into all wells
  6. Cover with a plastic plate cover and incubate at room temperature (18 to 25°C) for 30 min
  7. Repeat wash step 4.
  8. Add 100μl of ready-to-use TMB Substrate Solution into all wells
  9. Incubate in the dark for 10-20 minutes* at room temperature. Avoid direct exposure to light by wrapping the plate in aluminium foil
  10. Add 100μl of H2SO4:Stop Reagent into all wells

Typical Standard Curve


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