Lysozyme (Serum/urine/liquor) ELISA Assay

$595.00

The Eagle Biosciences Lysozyme (Serum/urine/liquor) ELISA Assay Kit is an enzyme immunoassay intended for the quan-titative determination of lysozyme in human serum, urine and liquor. For research use only. Not for use in diagnostic procedures.

SKU: KR6902 Categories: , ,

Lysozyme (Serum/urine/liquor) ELISA Assay

Lysozyme Serum/urine/liquor ELISA Assay is manufactured in Germany by Immundiagnostik

Size: 1×96 wells
Standard Range: 1.1-30 ng/ml
Incubation Time: 1h; 1h; 10-20min
Sample Type: Serum, urine, liquor
Sample Size: 50 µL
For Research Use Only
Controls Included


Assay Principle

The Lysozyme ELISA utilises the “sandwich” technique with two selected antibodies that recognise human lysozyme. Standards, controls and diluted samples, which are assayed for human lysozyme, are added into the wells of a micro plate coated with a high affine anti-human lysozyme antibody. During the first incubation step, lysozyme is bound by the immobilised antibody. Then a peroxidase-conjugated anti-human lysozyme antibody is added into each microtiter well and a “sandwich” of capture antibody – human lysozyme – peroxidase-conjugate is formed. Tetramethylbenzidine is used as peroxidase substrate. Finally, an acidic stop solution is added to terminate the enzymatic reaction. The color changes from blue to yellow. The intensity of the yellow colour is directly proportional to the concentration of lysozyme. A dose response curve of the absorbance unit (optical density, OD at 450 nm) vs. concentration is generated using the values obtained from the standards. Lysozyme, present in the samples, is determined directly from this curve.
Indications for Lysozyme
• Diagnosis and monitoring of Crohn’s disease
• Early diagnosis of rejection reactions in kidney transplantation cases
• Differential diagnosis and monitoring of leukosis
• Diagnosis and treatment monitoring of urinary tract infections in children
• Differential diagnosis between viral and bacterial meningitis in children
• Identification of sepsis in neonates


Related Products to Lysozyme ELISA

Lysozyme (Stool) ELISA Kit
Thymulin ELISA Assay
Thymosin beta-4 (ß4) ELISA

Additional Information

Assay Background


Lysozyme (muramidase) is a protein with a molecular weight of approx. 15 kDa and belongs to the group of alkaline glycosidases. Lysozyme is produced by granulo-cytes, monocytes and macrophages. The main source for faecal lysozyme are the intestinal granulocytes. Lysozyme can be detected in all cells of the inflammatory infiltrate during an acute attack of Crohn‘s disease. To some extent, lysozyme is also secreted actively by mononuclear cells into the bowel lumen.

Manual

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