Thymosin beta-4 (ß4) ELISA Assay Kit


The Eagle Biosciences Thymosin beta-4 (ß4) ELISA Assay Kit is an enzyme immunoassay intended for the quantitative determination of thymosin β4 in serum and thymus extract. For research use only. Not for use in diagnostic procedures.

SKU: KR9520 Categories: , ,

Thymosin beta-4 (ß4) ELISA Assay Kit

For Research Use Only

Size: 1×96 wells
Standard Range: 0.16-10 µg/ml
Incubation Time: 1 h, 1 h, 10-20 min
Sample Type: Serum, Thymus extract
Sample Size: 50 µL

Product manufactured in Germany by Immundiagnostik

Additional Information

Assay Background

The beta-thymosins are a family of highly conserved polar 5 kDa peptides originally thought to be thymus hormones. Further studies demonstrated that beta-thymosins are ubiquitous; they have been found in a variety of tissues and cell lines. The high-est concentrations have been detected in spleen, thymus, lung, and peritoneal macrophages.
Thymosin β4 binds monomeric actin in a 1:1 complex acting as an actin buffer, preventing polymerisation into actin filaments but supplying a pool of actin monomers when needed by the cell. Changes in the expression of thymosin β4 appear to be related to the differentiation of cells. Thymosin β4 is detected outside of cells in blood plasma and wound fluid. Several biological effects are attributed to thymosin β4, like induction of metallo-proteinases, chemotaxis, angiogenesis and inhibition of inflammation as well as inhibition of bone marrow stem cell proliferation.

Assay Procedure

This ELISA is designed for the quantitative determination of thymosin ß4 (Tß4) in serum and thymus preparations. The test principle is based on a competition between antigen in the sample or standards and the antigen coated on the wells of microplate. A peroxidase-conjugated antibody is used for detection and quantification, and tetramethylbenzidine (TMB) as a peroxidase substrate. The enzymatic reaction is terminated by acidic stop solution. The intensity of the colour is inversely proportional to the concentration of Tß4. A dose response curve of absorbance unit (optical density, OD at 450 nm) vs. concentration is generated using the values obtained from standard. Tß4, present in the samples, is determined directly from this curve.


Product Manual