The LBP ELISA Kit for a wide variety of species is to be used for the quantitative determination of LBP in cell culture supernatants, plasma or serum. Measurable species are amongst others: bovine, canine, human, monkey, rabbit, rat, sheep and swine. For quantification, human LBP is used as a reference standard. The Eagle Biosciences LBP ELISA Kit is for research use only and not for diagnostic or therapeutic procedures.


The LBP ELISA Kit is For Research Use Only

Sizes: 1×96 wells
Sensitivity: 1.6 ng/ml
Standard Range: 1.6 to 100 ng/ml
Incubation Time: 3.5 hours
Sample Type: Serum, plasma, cell culture supernatants
Sample Size: 100 µl
Alternative Names: Lipopolysaccharide Binding Protein

Assay Background for the LBP ELISA kit

Lipopolysaccharide (LPS) Binding Protein (LBP) is a type 1 acute phase protein that is constitutively produced by the liver and rapidly upregulated during the acute phase response. LBP plays a central role in the response to LPS. The protein catalyses the monomerization of LPS and its transfer to (s)CD14 and to lipoproteins. In this way LBP has both a role in the activation pathway of LPS: activation of monocytes by LPS leading to release of inflammatory mediators and in the neutralization of LPS i.e. the uptake of LPS by lipoprotein and subsequent clearing. The assay can be used to quantify functional LBP in tissue culture supernatants, plasma and serum samples of several species. In plasma of healthy individuals LBP is present at levels of approximately 10 μg/ml, which increase approximately 10-fold during acute phase responses.

Assay Principle

The LBP ELISA kit is a ready-to-use solid-phase enzyme-linked immunosorbent assay based on the sandwich principle with a working time of 3½ hours. The efficient format of 1 plate with twelve disposable 8-well strips allows free choice of batch size for the assay. Samples and standards are incubated in microtiter wells coated with antibo­dies recognizing LBP. Biotinylated LPS will bind to captured LBP. Streptavidin-peroxidase conjugate will bind to the biotinylated LPS. Streptavidin-peroxidase conjugate will react with the substrate, tetramethylbenzidine (TMB). The enzyme reaction is stopped by the addition of citric acid. The absorbance at 450 nm is measured with a spectrophotometer. A standard curve is obtained by plotting the absorbance (linear) versus the corresponding concentrations of the LBP standards (log). The LBP concentration of samples, which are run concurrently with the human LBP standards, can be determined from the human LBP standard curve.

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