Unconjugated Free Estriol Saliva Sensitive ELISA


The Unconjugated Free Estriol Saliva Sensitive ELISA Assay Kit is designed and validated for the quantitative measurement of free estriol in human saliva. The Unconjugated Free Estriol Saliva Sensitive ELISA is for research use only and not for use in diagnostic procedures.

SKU: UE332-K01 Categories: , , Tags: , ,

Unconjugated Free Estriol Saliva Sensitive ELISA

The Unconjugated Free Estriol Saliva Sensitive ELISA is For Research Use Only

Size: 1×96 wells
Sensitivity: 0.860 pg/ml
Incubation Time: 2 hours
Sample Type: Human Saliva
Sample Size: 50 µl

Controls Included

Product manufactured in the USA

Storage and Stability
When stored at 2° – 8°C, unopened reagents will retain activity until the expiration date. Do not use reagents beyond this date.
Use only reagents supplied with this kit. Do not interchange reagents with different lot numbers.
Opened reagents must be stored at 2° – 8°C.
Microtiter wells must be stored at 2° – 8°C. Once the foil bag has been opened, care should be taken to reseal tightly.
Opened kits retain activity for 28 days if stored as described above.
Expiration dates and lot numbers are printed on the labels.

Assay Background

Estriol is the weakest of the three major estrogens with a binding affinity for the estrogen receptor about 1/100 that of estradiol. Physiologically significant amounts of Estriol are only present during pregnancy or with supplementation. Estriol is available, mainly, as a custom compounded product and is one of the components of bi- or tri-estrogen creams usually in a ratio of estradiol to estrone to estriol 1:1:8. Salivary estriol median levels are <2 pg/ml in non-pregnant pre- and post-menopausal women as well as in men. These levels represent little to no physiologic estrogenic activity. In pregnancy, low levels of Estriol may indicate fetal distress and in Down Syndrome Estriol measurements are part of a screen for this condition.

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  • Canez, MS, LEE, KH, Olive, DL, Progestogens and Estrogens, Infertil Reproduct Med Clin No Amer 1992, 3: 59-78.
  • Yang, TS, Tsan, SH, Chang, SP, Ng, HT, Efficacy and Safety of Estriol Replacement Therapy for Climacteric Women, Chung Hua I Hsueh Tsa Chich (Taipei), 1995, 550 (5): 386-91.
  • McGregor, JA, Jackson, GM, Lachelin, GCl, et al., Salivary Estriol as Risk Assessment for Preterm Labor: A Prospective Trial, Am J Obstet Gynecol 1995, 173:4, 1337-1342.
  • Reis, F.M., D’Antona, D., and Petraglis, F. (2002). Predictive Values of Hormone Measurements in Maternal and Fetal Complications of Pregnancy. Endocrine Reviews, 23 (2) 230-257.

Additional Information

Assay Principle

The Salivary Free Estriol ELISA Assay kit is based on the competition principal and microplate separation. Estriol calibrators and unknown amounts of estriol in saliva samples compete with a fixed amount of estriol conjugated to horse radish peroxidase (Estriol-HRP) for binding sites with a rabbit estriol monoclonal antiserum bound to GARGG (goat anti-rabbit gamma globulin) coated wells of a microplate. After incubation, unbound components are washed away, enzyme substrate solution is added and a blue color formed. This reaction is stopped with an acid solution to produce a yellow color. The optical density is then read at 450 nm. The amount of Estriol-HRP detected is inversely proportional to the amount of estriol in a sample.


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