L-Arginine ELISA Assay Kit

$950.00

The L-Arginine ELISA Assay Kit is an enzyme immunoassay for the quantitative determination of L-Arginine (Arg) in serum, plasma or cell culture samples. The L-Arginine ELISA Assay Kit is for research use only and not for use in diagnostic procedures.

L-Arginine ELISA Assay Kit

L-Arginine ELISA Assay Kit Developed and Manufactured in France by ImmuSmol

Size: 1×96 wells
Sensitivity: 2.1 µM
Standard Range: 5.12-200 µM
Incubation Time: Overnight
Sample Type: Serum, Plasma and Cell Culture
Sample Size: 20µL
Alternative Names: Arginine ELISA, L-Arg,
For Research Use Only

Controls Included


Background

Enzyme Immunoassay for the quantitative determination of L-Arginine (Arg) in plasma samples. After extraction and derivatization Arginine is quantitatively determined by ELISA. The competitive ELISA uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The processed standards, controls and samples and the solid phase bound analyte compete for a fixed number of antiserum binding sites. When the system is in equilibrium, free antigen and free antigen-antiserum complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgG-peroxidase conjugate using TMB as a substrate. The reaction is monitored at 450 nm. Quantification of unknown samples is achieved by comparing their absorbance with a reference curve prepared with known standards.

Specificity: No significant cross-reactivity was observed with L-Homoarginine, Agmatine, L-Citrulline, L-Ornithine, ADMA or SDMA


Assay Principle

The competitive ELISA uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The processed standards, controls and samples and the solid phase bound analyte compete for a fixed number of antiserum binding sites. When the system is in equilibrium, free antigen and free antigen-antiserum complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgG-peroxidase conjugate using TMB as a substrate. The reaction is monitored at 450 nm. Quantification of unknown samples is achieved by comparing their absorbance with a reference curve prepared with known standards.


Products Related to L-Arginine ELISA Assay Kit

ADMA Arginine ELISA Assay Kit
Homoarginine ELISA Assay Kit
ADMA ELISA Assay Kit
SDMA ELISA Assay Kit

Additional Information

Assay Procedure


Allow all reagents and samples to reach room temperature and mix thoroughly by gentle inversion before use. Duplicate determinations are recommended. To do that, perform duplicates after derivatization (1 derivatization well = 2 competition wells).
It is recommended to number the strips of the microwell plate before usage to avoid any mix-up.
The binding of the antisera and of the enzyme conjugate and the activity of the enzyme are temperature dependent, and the absorbance values may vary if a thermostat is not used. The higher the temperature, the higher the extinction values will be. Corresponding variations also apply to the incubation times. The optimal temperature during the Enzyme Immunoassay is between 20 – 25 °C.

Typical Standard Curve


Documents

Product Manual