L-Arginine ELISA Assay Kit
L-Arginine ELISA Assay Kit Developed and Manufactured in France by ImmuSmol
Size: 1×96 wells
Sensitivity: 2.1 µM
Standard Range: 5.12-200 µM
Incubation Time: Overnight
Sample Type: Serum, Plasma and Cell Culture
Sample Size: 20µL
Alternative Names: Arginine ELISA, L-Arg,
For Research Use Only
Controls Included
Background
Enzyme Immunoassay for the quantitative determination of L-Arginine (Arg) in plasma samples. After extraction and derivatization Arginine is quantitatively determined by ELISA. The competitive ELISA uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The processed standards, controls and samples and the solid phase bound analyte compete for a fixed number of antiserum binding sites. When the system is in equilibrium, free antigen and free antigen-antiserum complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgG-peroxidase conjugate using TMB as a substrate. The reaction is monitored at 450 nm. Quantification of unknown samples is achieved by comparing their absorbance with a reference curve prepared with known standards.
Specificity: No significant cross-reactivity was observed with L-Homoarginine, Agmatine, L-Citrulline, L-Ornithine, ADMA or SDMA
Assay Principle
The competitive ELISA uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The processed standards, controls and samples and the solid phase bound analyte compete for a fixed number of antiserum binding sites. When the system is in equilibrium, free antigen and free antigen-antiserum complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgG-peroxidase conjugate using TMB as a substrate. The reaction is monitored at 450 nm. Quantification of unknown samples is achieved by comparing their absorbance with a reference curve prepared with known standards.
Products Related to L-Arginine ELISA Assay Kit
ADMA Arginine ELISA Assay Kit
Homoarginine ELISA Assay Kit
ADMA ELISA Assay Kit
SDMA ELISA Assay Kit
