Anti-Factor H ELISA Assay
Anti-Factor H ELISA Assay Developed and Manufactured Medipan
Size: 1×48 wells
Sensitivity: 1.0 U/m
Dynamic Range: 10 – 300 U/mL
Incubation Time: 2 hours
Sample Type: Serum
Sample Size: 10 µL
Alternative Names: Human Anti-Factor H, Human Factor H Antibody, Complement Factor H Antibody, Anti-Factor H IgG
For Research Use Only
Analyzing 93 sera of healthy donors, 4 samples have been found above the cut-off, leading to a specificity of 95.7%.
The Anti-Factor H ELISA Assay Kit is an enzyme immunoassay for the quantitative determination of IgG antibodies to complement factor H. The antibodies of the calibrators, controls, and diluted patient samples react with human recombinant complement factor H immobilized on the solid phase of microtiter plates. Following an incubation period of 60 min at room temperature (RT), unbound serum components are removed by a wash step. The bound IgG antibodies react specifically with anti-human-IgG conjugated to horseradish peroxidase (HRP) within the incubation period of 30 min at RT. Excessive conjugate is separated from the solid-phase immune complexes by the following wash step. HRP converts the colorless substrate solution of 3,3’,5,5’-tetramethyl-benzidine (TMB) added into a blue product. The enzyme reaction is stopped by dispensing an acidic solution into the wells after 15 min at RT turning the solution from blue to yellow. The optical density (OD) of the solution at 450 nm is directly proportional to the amount of specific antibodies bound. The standard curve is established by plotting the antibody concentrations of the calibrators (x-axis) and their corresponding OD values (y-axis) measured. The concentration of antibodies of the specimen is directly read off the standard curve.