IGFBP-3 ELISA Assay Kit

$570.00

The IGFBP-3 ELISA Assay kit quantifies IGFBP-3 in human Serum, Heparin or EDTA plasma. The Human IGFBP-3 ELISA Assay kit is for Research Use Only.

SKU: E03A Categories: , ,

IGFBP-3 ELISA Assay Kit

The IGFBP-3 ELISA Assay Kit is For Research Use Only

Size: 1×96 wells
Sensitivity: 0.03 ng/mL
Incubation Time: 2.5 hours
Sample Type: Serum, EDTA or Heparin Plasma
Sample Size: 10 µL
Alternative Name: Insulin-Like Growth Factor-3 ELISA, Human IGFBP-3 ELISA

Manufactured by Mediagnost


Assay Principle

The IGFBP-3 ELISA is a so-called Sandwich-Assay. It utilizes two specific antibodies of high affinity. First the IGFBP-3 in the sample binds to the immobilized antibody on the microtiter plate. In the following step, the complex of biotinylated anti-IGFBP-3-Antibody and Streptavidin-Peroxidase binds in turn to the immobilised IGFBP-3. Subsequently, the peroxidase catalyzes an enzymatic reaction resulting in a blue coloration. The intensity of the blue color depends on the IGFBP-3 content of the sample. The reaction is stopped by the addition of stop solution and color intensity is quantified by measuring the absorption.


Linearity

Linearity was proven by dilution of three different serum samples with known IGFBP-3 concentration. The IGFBP-3 concentration of the diluted sample was measured and compared with the concentration expected. Results of linear regression analysis are shown in Figure 2. None of IGFBP-3 concentrations of the dilutions (1:125 to 1:2000) deviated more than 20% of the expected value (max. -17%).

Sample stability
In firmly closable sample vials

  • Storage at 20-25°C: 3 days
  • Storage at -20° C: min. 2 years
  • Freeze-thaw cycles max. 10

The storage of samples over a period of 2 years at -20°C, showed no influence on the reading. Freezing and thawing of samples should be minimized. 10 Freezing-Thawing showed no effect on samples


Related Products

IGFBP-2 ELISA Kit
Human IGFBP-1 ELISA Assay
IGFBP-6 ELISA

Additional Information

Assay Background


Insulin-like growth factors (IGF)-I and -II are bound to specific binding proteins (IGFBPs) in the circulation. To date, at least six binding proteins can be distinguished on the basis of their amino acid sequence. They are designated as IGFBP-1, IGFBP-2 to IGPBP-7 (1-2). The predominating IGFBP in blood is IGFBP-3, which largely determines the total IGF-I and IGF-II concentration. In contrast to the other binding proteins, IGFBP-3 has the property to associate with an acid-labile non-binding subunit (ALS) after binding of either IGF-I or IGF-II (3-5). Most of the IGFBP-3 in plasma is present as the high molecular weight ternary complex, however, small amounts of free IGFBP-3 are also found (6,7).

The development of specific immunoassays for IGFBP-3, recognizing the complete high molecular weight complex, provided new in-sights into ternary complex regulation (6-9).  Several factors besides GH influence IGFBP-3 levels: age including sexual development, nutrition, hypothyroidism, diabetes mellitus, liver function and kidney function.  Measurement over 24 hours revealed constant circadian levels (12,13).

IGFBP-3 ELISA Assay Kit

Documents

Product Documents