IGFBP-3 ELISA Assay Kit
The IGFBP-3 ELISA Assay Kit is For Research Use Only
Size: 1×96 wells
Sensitivity: 0.03 ng/mL
Incubation Time: 2.5 hours
Sample Type: Serum, EDTA or Heparin Plasma
Sample Size: 10 µL
Alternative Name: Insulin-Like Growth Factor-3 ELISA, Human IGFBP-3 ELISA
Manufactured by Mediagnost
The IGFBP-3 ELISA is a so-called Sandwich-Assay. It utilizes two specific antibodies of high affinity. First the IGFBP-3 in the sample binds to the immobilized antibody on the microtiter plate. In the following step, the complex of biotinylated anti-IGFBP-3-Antibody and Streptavidin-Peroxidase binds in turn to the immobilised IGFBP-3. Subsequently, the peroxidase catalyzes an enzymatic reaction resulting in a blue coloration. The intensity of the blue color depends on the IGFBP-3 content of the sample. The reaction is stopped by the addition of stop solution and color intensity is quantified by measuring the absorption.
Linearity was proven by dilution of three different serum samples with known IGFBP-3 concentration. The IGFBP-3 concentration of the diluted sample was measured and compared with the concentration expected. Results of linear regression analysis are shown in Figure 2. None of IGFBP-3 concentrations of the dilutions (1:125 to 1:2000) deviated more than 20% of the expected value (max. -17%).
In firmly closable sample vials
- Storage at 20-25°C: 3 days
- Storage at -20° C: min. 2 years
- Freeze-thaw cycles max. 10
The storage of samples over a period of 2 years at -20°C, showed no influence on the reading. Freezing and thawing of samples should be minimized. 10 Freezing-Thawing showed no effect on samples