IGFBP-2 ELISA Assay Kit

$740.00

The IGFBP-2 ELISA Assay Kit is for the Measurement of human IGFBP-2 in human serum, EDTA-plasma, cerebrospinal fluid, breast milk, amniotic fluid, saliva and in cell culture medium. The IGFBP-2 ELISA Assay Kit is for Research Use Only.

SKU: E05 Categories: , ,

IGFBP-2 ELISA Assay Kit

The IGFBP-2 ELISA Assay Kit is For Research Use Only

Size: 1×96 wells
Sensitivity: 0.2 ng/ml
Standard Range: 2 – 80 ng/ml
Incubation Time: 2 hours
Sample Type: Serum, Plasma, Biological Fluids, Cell Culture
Sample Size: 15 µL

Manufactured by Mediagnost

Assay Principle

The Eagle Biosciences ELISA for IGFBP-2 E05 is a so-called Sandwich-Assay using two specific and high-affinity antibodies. The IGFBP-2 in the samples binds to the first antibody coated on the microtiter plate. In the following step, the second specific anti-IGFBP-2-Antibody binds in turn to the immobilized IGFBP-2. The second antibody is biotinylated and will be applied in a mixture with a Streptavidin-Peroxidase-Enzyme Conjugate. In the closing substrate reaction, the turn of the colour will be catalysed quantitatively depending on the IGFBP-2-level of the samples.

Related Products

Human IGFBP-1 ELISA Assay
IGFBP-3 ELISA Kit

Additional Information

Assay Background

Insulin-like growth factors (IGFs) regulate the proliferation, differentiation, apoptosis, cell adhesion and metabolism in various tissues and cell types. The IGF-I, which is produced mainly in liver under the influence of growth hormone (GH), regulates as hormone the linear growth of the bones and the process of sexual maturity, while IGF-II is mainly a growth factor of fetal tissue. The biological actions of IGF over the IGF-Type-I receptor are modulated variably through the IGF binding proteins (IGFBP-1 to-6). IGFBP-2 is, after IGFBP-3, the second most frequent IGFBP in the human blood. IGFs, especially tumor typical pro-IGF-forms and hormones regulate the expression of IGFBP-2, GH effect is thereby inhibiting. At cellular level IGFBP-2 seems to stimulate the proliferation and dissemination of solid tumors via an IGF-independent mechanism.

IGFBP-2 is an unglycosylated polypeptide of 31.3 kDa, which forms binary IGF-complexes and shows no circadian rhythm in the circulation. The serum concentration of IGFBP-2 increases in fasting, after major surgery and after trauma, but the increase of the concentration is most intensive in malignant diseases. The correlation of the IGFBP-2 level to the degree of progression is a striking feature in various tumor types as is the normalization of the IGFBP-serum levels after remission. The IGFBP-2 concentration is age-dependent in blood.

Typical Standard Curve

IGFBP-2 ELISA Assay Kit

Specificity

This assay is specific for human IGFBP-2, only a low degree of cross-reactions was found with dog, horse, donkey, cat and goat. No cross-reactivity was found with pig, bovine, rabbit, mouse, chicken, rat, guinea pig, sheep.  There was no cross-reactivity with IGFBP-1 and IGFBP-3.

Package Inserts

Please note: All documents above are for reference use only and should not be used in place of the documents included with this physical product. If digital copies are needed, please contact us.

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