The IGFBP-2 ELISA Assay Kit is for the Measurement of human IGFBP-2 in human serum, EDTA-plasma, cerebrospinal fluid, breast milk, amniotic fluid, saliva and in cell culture medium. The IGFBP-2 ELISA Assay Kit is for Research Use Only.


The IGFBP-2 ELISA Assay Kit is For Research Use Only

Size: 1×96 wells
Sensitivity: 0.2 ng/ml
Standard Range: 2 – 80 ng/ml
Incubation Time: 2 hours
Sample Type: Serum, Plasma, Biological Fluids, Cell Culture
Sample Size: 15 µL

Manufactured by Mediagnost

Assay Principle

The Eagle Biosciences ELISA for IGFBP-2 E05 is a so-called Sandwich-Assay using two specific and high-affinity antibodies. The IGFBP-2 in the samples binds to the first antibody coated on the microtiter plate. In the following step, the second specific anti-IGFBP-2-Antibody binds in turn to the immobilized IGFBP-2. The second antibody is biotinylated and will be applied in a mixture with a Streptavidin-Peroxidase-Enzyme Conjugate. In the closing substrate reaction, the turn of the colour will be catalysed quantitatively depending on the IGFBP-2-level of the samples.

Related Products

Human IGFBP-1 ELISA Assay
Human IGFBP-2 Recombinant Protein, Biotin-labeled

Additional Information

Assay Background

Insulin-like growth factors (IGFs) regulate the proliferation, differentiation, apoptosis, cell adhesion and metabolism in various tissues and cell types. The IGF-I, which is produced mainly in liver under the influence of growth hormone (GH), regulates as hormone the linear growth of the bones and the process of sexual maturity, while IGF-II is mainly a growth factor of fetal tissue. The biological actions of IGF over the IGF-Type-I receptor are modulated variably through the IGF binding proteins (IGFBP-1 to-6). IGFBP-2 is, after IGFBP-3, the second most frequent IGFBP in the human blood. IGFs, especially tumor typical pro-IGF-forms and hormones regulate the expression of IGFBP-2, GH effect is thereby inhibiting. At cellular level IGFBP-2 seems to stimulate the proliferation and dissemination of solid tumors via an IGF-independent mechanism.

IGFBP-2 is an unglycosylated polypeptide of 31.3 kDa, which forms binary IGF-complexes and shows no circadian rhythm in the circulation. The serum concentration of IGFBP-2 increases in fasting, after major surgery and after trauma, but the increase of the concentration is most intensive in malignant diseases. The correlation of the IGFBP-2 level to the degree of progression is a striking feature in various tumor types as is the normalization of the IGFBP-serum levels after remission. The IGFBP-2 concentration is age-dependent in blood.

Typical Standard Curve



This assay is specific for human IGFBP-2, only a low degree of cross-reactions was found with dog, horse, donkey, cat and goat. No cross-reactivity was found with pig, bovine, rabbit, mouse, chicken, rat, guinea pig, sheep.  There was no cross-reactivity with IGFBP-1 and IGFBP-3.

Package Inserts

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