IGFBP-6 ELISA Assay Kit
The IGFBP-6 ELISA Assay Kit is For Research Use Only
Size: 1×96 wells
Sensitivity: 0.026 ng/mL
Dynamic Range: 0.1 – 10 ng/ml
Incubation Time: 3 hours
Sample Type: Serum, Plasma, Biological Fluids
Sample Size: 10 ul
IGFBP-6 ELISA Assay Kit is manufactured by Mediagnost
The Eagle Biosciences IGFBP-6 ELISA assay is based on polyclonal antibodies and recombinant IGFBP-6, expressed in eukaryotic cells. This kit for IGFBP-6 is a so-called Sandwich-Assay. It utilizes two specific antibodies of high affinity. First the IGFBP-6 in the sample binds to the immobilized antibody on the microtiter plate. In a two-step sequence, the biotin-conjugated anti-IGFBP-6-Antibody and the streptavidin-peroxidase are bound. Subsequently, the peroxidase catalyzes an enzymatic reaction resulting in a blue coloration. The intensity of the blue color depends on the analyte content of the sample. The reaction is stopped by the addition of stop solution and color intensity (yellow) is quantified by measuring the absorption.
The IGFBP-6 ELISA,allows secure and reproducible measurement of IGFBP-6 in human body fluids and is a suitable tool for the investigation of IGFBP-6 as a biomarker in growth, cancer and bone metabolism. In a preliminary study IGFBP-6 was measured in serum of healthy blood donors and mean concentration of 204 ng/mL was detected (Range: 73-367, n=20).
IGFBP-6 was measured in blood samples of healthy blood donors. Measured mean is 204 ng/mL (Range: 73-367).