Human Zinc Transporter 8 (ZNT8) ELISA Assay Kit
The Human Zinc Transporter 8 (ZNT8) ELISA Assay Kit is For Research Use Only
Size: 1 x 96 wells
Calibrator Range: 0.312 – 20 ng/mL
Sensitivity: 0.188 ng/mL
Sample Size: 100 uL
Sample Type: Serum, Plasma, Urine, Cell Culture Supernatant, Tissue Samples
Incubation Time: 3 hours 10 minutes
Assay Background
Zinc Transporter 8 (ZNT8) is a protein that plays a critical role in the regulation of insulin secretion in pancreatic beta cells. ZNT8 is primarily located in the insulin-secretory granules of these cells. It functions as a zinc transporter, facilitating the uptake of zinc into insulin vesicles. Zinc is essential for the proper folding and stability of insulin molecules within the vesicles. The controlled release of insulin in response to changes in blood glucose levels is a crucial aspect of glucose homeostasis, and ZNT8 contributes to this process by ensuring the availability of zinc for insulin storage and secretion. Mutations in the ZNT8 gene have been associated with an increased risk of developing autoimmune type 1 diabetes, as the immune system may target ZNT8 along with other proteins in pancreatic beta cells. Understanding the function of ZNT8 is pivotal in unraveling the intricate mechanisms underlying insulin regulation and the pathophysiology of diabetes.
Assay Principle
The method employs sandwich ELISA technique. Monoclonal antibodies are pre-coated onto microwells. Samples and standards are pipetted into microwells and Human Zinc Transporter 8, ZNT8 present in the sample are bound by the antibodies. Biotin labeled antibody is added and followed by Streptavidin-HRP is pipetted and incubated to form a complex. After washing microwells in order to remove any non-specific binding, the substrate solution (TMB) is added to microwells and color develops proportionally to the amount of Human Zinc Transporter 8, ZNT8 in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.
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