Human Programmed Death Ligand-1 (PD-L1 / CD274) ELISA Assay Kit

$490.00

The Human Programmed Death Ligand-1 (PD-L1 / CD274) ELISA Assay Kit is used as an analytical tool for quantitative determination of Programmed Death Ligand-1 (PD-L1 / CD274) in serum, plasma, and other biological samples. The Human Programmed Death Ligand-1 (PD-L1 / CD274) ELISA Assay Kit is for research use only and not to be used for diagnostic procedures.

Human Programmed Death Ligand-1 (PD-L1 / CD274) ELISA Assay Kit

The Human Programmed Death Ligand-1 (PD-L1 / CD274) ELISA Assay Kit is For Research Use Only

Size: 1 x 96 wells
Calibrator Range: 12.5 – 600 pg/mL
Sensitivity: 12.5 pg/mL
Sample Size: 100 uL
Sample Type: Serum, Plasma, Urine, Cell Culture Supernatant, Tissue Samples
Incubation Time: 3 hours 30 minutes


Assay Principle

The method employs sandwich ELISA technique. Monoclonal antibodies are pre-coated onto microwells. Samples and standards are pipetted into microwells and Human Programmed Death Ligand-1, PD-L1 / CD274 present in the sample are bound by the antibodies. HRP conjugated PD-L1 is added to the wells and incubated and then washed. After washing microwells in order to remove any non-specific binding, the substrate solution (TMB) is added to microwells and color develops proportionally to the amount of Human Programmed Death Ligand-1, PD-L1/CD274 in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.

Handling / Storage

  1. All reagents should be stored as indicated on the component label.
  2. All the reagents and wash solutions should be used within 12 months from manufacturing date.
  3. Before using, bring all components to room temperature (18-25ºC). Upon assay completion ensure all components of the kit are returned to appropriate storage conditions.
  4. The Substrate is light-sensitive and should be protected from direct sunlight or UV sources.

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Documents

Product Manual


Please note: All documents above are for reference use only and should not be used in place of the documents included with this physical product. If digital copies are needed, please contact us.