Protein A ELISA Assay Kit
The Protein A ELISA Assay Kit is For Research Use Only
Size: 1 x 96 wells
Calibrator Range: 62 – 4000 pg/mL
Sensitivity: 64 pg/mL
Sample Size: 100 uL
Sample Type: Cell Culture Supernatant and other Biological Preparations
Incubation Time: 1 hour 40 minutes
Controls Not Included
A variety of antibodies are used as therapeutics agent as well as in pharmaceuticals. These antibodies are generally purified through protein A column chromatography. This method of purification has the potential with contamination of protein A, which might get leached out of the column and co-eluted with purified antibody. This may result in adverse toxic or immunological reactions that ultimately affect the efficacy of the product antibody. The highly sensitive Protein A ELISA is a quantitative, simple and will aid in monitoring the purification process as well as screening product prior to its use.
This assay is based on the principle of Sandwich ELISA. Samples containing Protein A are reacted with anti-Protein A antibody coated on the microtiter plates. The wells are washed to remove any unbound reactants as per the wash procedure. Anti-Protein A HRP antibody is added to the wells and incubated. . The wells are washed to remove any unbound reactants as per the wash procedure. simultaneously in the microtiter wells already coated with affinity purified capture anti-Protein A antibody. The substrate 3,3’,5,5’ Tetramethyl Benzidine is then reacted. The amount of hydrolyzed substrate is read on a microtiter plate reader and it is directly proportional to the concentration of Protein A present.