Human Pro-IGF-II (1-156)


The Eagle Bioscience’s Human Pro-IGF-II (1-156) is for research use only and not to be used for diagnostic procedures.

SKU: AHU020 Categories: ,

 Human Pro-IGF-II (1-156) 

The Eagle Bioscience’s Human Pro-IGF-II (1-156) is for research use only and not to be used for diagnostic procedures.

Product Specifications


IGF-II is produced in E.coli using a patented expression system.


The IGF-II gene encodes a single transcript which results in a 156 amino acid protein, including an 89 amino acid E-domain. Pro-IGF-II is converted to mature IGF-II by sequential cleavage at amino acids 104, 87 and 67. Pro-IGF-II contains sites of O-linked glycosylation. Differential glycosylation and cleavage within the E-domain can therefore result in multiple pro-IGF-II isoforms. The pro-IGF-II proteins make up 10-20% of circulating IGF-II. Pro-IGF-II proteins are secreted by some tumour cell lines and levels are elevated in non-islet cell tumour hypoglycaemia. 

  • Purity: >85 % (as determined by HPLC analysis)
  • Quantity: 20 µg
  • Molecular Weight: The final product contains three major isoforms of pro-IGF-II corresponding to molecular weights of 16.1, 17.0 and 17.6 kDa. These species correspond to the full length 156 amino acid protein and two smaller species corresponding to C-terminal truncations of approximately 5 and 15 residues which result from the purification process
  • N-terminal sequence analysis of recombinant product: 5 residues >95% single sequence
  • Biological Activity: Stimulation of protein synthesis in rat L6 myoblasts (ED50 < 220 ng/ml)
  • Endotoxin (LAL): <0.1 EU/ug
  • Storage/Stability: At least 2 years at 2-4°C (lyophilized)
  • Appearance: White Powder
  • State: Dried from 0.1 M acetic acid under dry nitrogen at a slight vacuum (-25kPa)

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Package Inserts

Please note: All documents above are for reference use only and should not be used in place of the documents included with this physical product. If digital copies are needed, please contact us.

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