Human [Leu24] [Ala31] IGF-I

$170.00

The Eagle Bioscience’s Human [Leu24] [Ala31] IGF-I is for research use only and not to be used for diagnostic procedures.

SKU: ADU020 Categories: ,

Human [Leu24] [Ala31] IGF-I

The Eagle Bioscience’s Human [Leu24] [Ala31] IGF-I is for research use only and not to be used for diagnostic procedures.


Product Specifications

Source:

IGF-I is produced in E.coli using a patented expression system and the IGF-I is correctly folded and purified by several chromatography steps to yield Receptor Grade IGF-I.

Description:

Human [Leu24][Ala31]insulin-like Growth Factor-I ([Leu24][Ala31]IGF-I) is an analogue of human IGF-I which has an Leucine substitution in place of Tyrosine at position 24 and an Alanine substitution in place of Tyrosine at position 31 of the human IGF-I sequence. [Leu24][Ala31]IGF-I has greatly reduced binding to the type 1 IGF receptor and to the insulin receptor compared to IGF-I, [Leu24] IGF-I and [Leu60] IGF-I. However, [Leu24] [Ala31] IGF-I has normal binding to acid-stripped human serum binding proteins. Consequently, [Leu24] [Ala31] IGF-I is an extremely useful analogue of IGF-I for investigating IGF actions independent of binding to the type 1 IGF receptor 

  • Purity: >95 % (as determined by HPLC analysis)
  • Quantity: 20 µg
  • Molecular Weight: 7507 daltons – confirmed by Mass Spectrometry
  • N-terminal sequence analysis of recombinant product: Analysis of 5 residues
  • Biological Activity: IGF type 1 receptor binding assay: (ED50 > 50 ng/ml) IGF binding protein assay: (ED50 < 10 ng/ml) Stimulation of protein synthesis in rat L6 myoblasts: (ED50 > 2000 ng/ml)
  • Endotoxin (LAL): <0.1 EU/ug
  • Storage/Stability: At least 2 years at 2-4°C (lyophilized)
  • Appearance: White Powder
  • State: Dried from 0.1 M acetic acid under dry nitrogen at a slight vacuum (-25kPa)

Related Products

Human IGF-I (Media Grade)
IGF-I ELISA Assay
Biotinylated Human Des IGF-1

Documents

Product Manual


Please note: All documents above are for reference use only and should not be used in place of the documents included with this physical product. If digital copies are needed, please contact us.

Product Citations