Human [Ala31] IGF-I


The Eagle Bioscience’s Human [Ala31] IGF-I is for research use only and not to be used for diagnostic procedures.

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Human [Ala31] IGF-I

The Eagle Bioscience’s Human [Ala31] IGF-I is for research use only and not to be used for diagnostic procedures.

Product Specifications


IGF-I is produced in E.coli using a patented expression system and the IGF-I is correctly folded and purified by several chromatography steps to yield Receptor Grade IGF-I.


Human [Ala31] insulin-like Growth Factor-I ([Ala31]IGF-I) is an analogue of human IGF-I which has an Alanine substitution in place of Tyrosine at position 31 of the human IGF-I sequence. [Ala31]IGF-I has normal binding to acid-stripped human serum binding proteins but has reduced binding to the type 1 IGF receptor and to the insulin receptor compared to IGF-I. It has a slightly higher affinity for the type 1 IGF receptor than [Leu24]IGF-I and [Leu60]IGF-I and is able to elicit a weak biological response through this receptor. It is a useful analogue for studying the balance of effects of IGF-I through the type 1 receptor and IGF binding proteins. 

  • Purity: >95 % (as determined by HPLC analysis)
  • Quantity: 100 µg
  • Molecular Weight: 7557 Daltons – confirmed by Mass Spectrometry
  • N-terminal sequence analysis of recombinant product: 5 residues >95% single sequence
  • Biological Activity: Stimulation of protein synthesis in rat L6 myoblasts(ED50 < 50 ng/ml) IGF type 1 receptor binding assay (ED50 > 10 ng/ml) IGF binding protein assay: ED50 < 10 ng/ml)
  • Endotoxin (LAL): <0.1 EU/ug
  • Storage/Stability: At least 2 years at 2-4°C (lyophilized)
  • Appearance: White Powder
  • State: Dried from 0.1 M acetic acid under dry nitrogen at a slight vacuum (-25kPa)

Related Products

Rodent IGF-I ELISA Assay
Human Arg3 IGF-1 Biotin

Package Inserts

Please note: All documents above are for reference use only and should not be used in place of the documents included with this physical product. If digital copies are needed, please contact us.

Product Citations