Human Neuregulin-1/NRG1-beta1 ELISA Kit

$450.00

The Eagle Biosciences Human Neuregulin-1/NRG1-beta1 ELISA Kit is intended for the quantitation of Human NRG1 concentrations in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). The Eagle Biosciences Human Neuregulin-1/NRG1-beta1 ELISA Kit is for research use only and not for diagnostic or therapeutic procedures.

SKU: NRG31-K01 Categories: , ,

Human Neuregulin-1/NRG1-beta1 ELISA Kit

For Research Use Only

Size: 1×96 wells
Sensitivity: <10pg/ml
Standard Range: 62.5pg/ml-4000pg/ml
Incubation Time: 2 hours 40 minutes
Sample Type: Cell culture supernates, cell lysates, serum and plasma (heparin, EDTA)
Sample Size: 100 µl

Additional Information

Assay Background


Neuregulin 1 or NRG1 is a protein that in humans is encoded by the NRG1 gene. It is mapped to 8p12. NRG1 is one of four proteins in the neuregulin family that act on the EGFR family of receptors. The protein encoded by this gene is a membrane glycoprotein that mediates cell-cell signaling and plays a critical role in the growth and development of multiple organ systems. Dysregulation of this gene has been linked to diseases such as cancer, schizophrenia, and bipolar disorder (BPD). It is also essential for the normal development of the nervous system and the heart. Furthermore, it was originally identified as a 44-kD glycoprotein that interacts with the NEU/ERBB2 receptor tyrosine kinase to increase its phosphorylation on tyrosine residues.

Assay Principle


The Human NRG1 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human NRG1 with a 96-well strip plate that is pre-coated with antibody specific for NRG1. The detection antibody is a biotinylated antibody specific for NRG1. The capture antibody is monoclonal antibody from mouse, the detection antibody is polyclonal antibody from goat. The kit contains recombinant Human NRG1 with immunogen: Expression system for standard: E.coli; Immunogen sequence: S2-K246. The kit is analytically validated with ready to use reagents. To measure Human NRG1, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Human NRG1 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells’ readings against the standard curve to determine the concentration of Human NRG1 in the sample.

Typical Standard Curve


Manual

Product Manual