Cancer Biomarker ELISA Assay

Human KLK13 ELISA Kit

$890.00

The Human KLK13 ELISA Kit is intended for the quantitation of Human KLK13 concentrations in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). The Eagle Biosciences Human KLK13 ELISA Kit is for research use only and not for diagnostic or therapeutic procedures.

SKU: KL331-K01 Categories: , ,

Human KLK13 ELISA Kit

The Human KLK13 ELISA Kit is For Research Use Only

Size: 1×96 wells
Sensitivity: <10pg/ml
Standard Range: 156pg/ml-10000pg/ml
Incubation Time: 2 hours 40 minutes
Sample Type: Cell culture supernates, cell lysates, serum and plasma(heparin, EDTA)
Sample Size: 100 µl
Alternative Name: Kallikrien-13

Assay Principle

The Human KLK13 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human KLK13 with a 96-well strip plate that is pre-coated with antibody specific for KLK13. The detection antibody is a biotinylated antibody specific for KLK13. The capture antibody is monoclonal antibody from mouse, the detection antibody is polyclonal antibody from goat. The kit contains recombinant Human KLK13 with immunogen: Expression system for standard: NSO, Immunogen sequence: G17-Q277. The kit is analytically validated with ready to use reagents. To measure Human KLK13, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Human KLK13 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells’ readings against the standard curve to determine the concentration of Human KLK13 in the sample.

Related Products

Human Kallikrein-14 ELISA Kit
Human KLK12 ELISA Kit
Human KLK11 ELISA Kit

Additional Information

Assay Background

Kallikrein-13 is a protein that in humans is encoded by the KLK13 gene. It belongs to the kallikrein subgroup of serine proteases, which have diverse physiologic functions in many tissues. By genomic sequence analysis, KLK13 gene is mapped in a 300-kb region on chromosome 19q13.3-q13.4. It has been shown that recombinant hK13 produced in yeast can cleave synthetic peptides after the arginine residue and some extracellular matrix components. However, its exact physiological substrates and functions remain obscure. Despite the lack of knowledge on the physiological function of hK13, several studies have demonstrated that hK13 is implicated with cancer of the breast and ovary and it can serve as a favorable prognostic biomarker for these malignancies.

Typical Standard Curve

Human KLK13 ELISA Kit

Documents

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