Human GFRa-4 Chicken Polyclonal Antibody

$535.00

Recombinant His-tagged human GFRa-4 protein produced using CHO-based Icosagen Cell factory Ltd. proprietary suspension cell line. For production of hGFRa-4, glycosylphosphatidyl-inositol GPI-anchor was removed and protein was secreted to the cell culture supernatant. Protein was purified by Ni-affinity chromatography following gel-filtration from cell culture supernatant.

SKU: 308-100 Categories: ,

Human GFRa-4 Chicken Polyclonal Antibody

The Human GFRa-4 Chicken Polyclonal Antibody is For Research Use Only

Immunogen: Human GFRa-4
Uniprot ID: Q9GZZ7
Alternative Names: GDNF receptor alpha-4, Persephin receptor
Clonality: Chicken polyclonal
Class: IgY
Reactivity: Human GFRa-4, doesn’t cross-react with hGFRa-1, hGFRa-2, hGFRa-3
Application: ELISA, WB, IF
Purification: GFRα-4 affinity purified
Buffer: PBS pH 7.4
Concentration: 1 mg/ml
Unit Size: 100 µg
Related Products: Mono- and polyclonal antibodies to human neural growth factors and neural growth factor receptors.
Shipping: This product is shipped in non-frozen liquid form in ambient conditions.
Storage: Store at – 20 or -70 °C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles as product degradation may result.
Alternative Names: Growth Factor Receptor a-4


Protocol: Western immunoblotting, recommended dilution  1:5 000 to 1:10 000; ELISA: 1:160 000 to 1:320 000, IF 1:1000 to 1:3000 Polyclonal antibody working titer has to be established practically for each particular antigen and assay format.

Background: The GFRα-4 belongs to the GDNF receptor family. It is a glycosyl-phosphatidylinositol (GPI)-linked cell surface receptor for persephin, and mediates activation of the RET tyrosine kinase receptor.


Related Products

Human GFRa-1 Chicken Polyclonal Antibody
Human GFRa-2 Chicken Polyclonal Antibody
Human GFRa-3 Chicken Polyclonal Antibody (Isoptype: IgY)
Product Developed and Manufactured by Icosagen

Additional Information

Figure 1:

 

 

 

 

 

 

 

IF detection of human GFRα-4 expressed in U2OS cells. GFRα-4 was visualized using Chicken polyclonal antibody to human GFRα-4 (dilution 1:2000) and Goat anti-chicken IgY – H&L DyLight 550 (Abcam, ab96952) (dilution 1:200) as secondary antibody. For nuclear staining DAPI was used. ArrayScan VTI platform (Thermo Scientific) was used for image acquisition (10x objective). Composite picture was generated using pseudocolors green for GFRα-4 specific signal and blue for nuclei.

Figure 2:

 

 

 

 

 

Western-Blot detection of human GFRα-4, using Chicken polyclonal antibody to human GFRα-4. Protein was expressed by CHO cell culture. 100 ng of purified protein was loaded per lane. Line 1. hGFRα-4. Primary antibody dilution 1:5000 was used. Line 2. Prestained protein ladder (Naxo 8003). Rabbit anti-chicken antibody, HRP conjugate (903-100, Icosagen) was used as secondary antibody.

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