Human GFRa-3 Chicken Polyclonal Antibody (Isoptype: IgY)
For Research Use Only
Immunogen: Human GFRa-3
Uniprot ID: O60609
Alternative Names: GDNF receptor alpha-3
Clonality: Chicken polyclonal
Reactivity: Human GFRa-3,doesn’t cross-react with hGFRa-1, hGFRa-2, hGFRa-4
Application: ELISA, WB, IF
Purification: GFRα-3 affinity purified
Buffer: Concentrated ammonium sulphate in PBS pH 7.4
Concentration: 1 mg/ml
Unit Size: 100 µg
Related Products: Mono- and polyclonal antibodies to human growth factors and neural growth factor receptors.
Shipping: This product is shipped in non-frozen liquid form in ambient conditions.
Storage: Store at +4°C upon receipt. As product is (NH4)2SO4 precipitate, mix well by pipetting or vortexing prior use
Recombinant His-tagged human GFRa-3 protein produced using CHO-based Icosagen Cell factory Ltd. proprietary suspension cell line. For production of hGFRa-3, glycosylphosphatidyl-inositol GPI-anchor was removed and protein was secreted to the cell culture supernatant. Protein was purified by Ni-affinity chromatography following gel-filtration from cell culture supernatant.
Western immunoblotting, recommended dilution 1:5 000 to 1:10 000; ELISA: 1:160 000 to 1:320 000, IF 1:1500 to 1:3000. Polyclonal antibody working titer has to be established practically for each particular antigen and assay format.
The GFRa-3 is a glycosylphosphatidylinositol(GPI)-linked cell surface receptor and a member of the GDNF receptor family. It forms a signaling receptor complex with RET tyrosine kinase receptor and binds the ligand, artemin.