Eagle Bio Antibodies

Human GFRa-1 Chicken Polyclonal Antibody

$610.00

Recombinant His-tagged human GFRa-1 protein produced using CHO-based Icosagen Cell factory Ltd. proprietary suspension cell line. For production of hGFRa-1, glycosylphosphatidyl-inositol GPI-anchor was removed and protein was secreted to the cell culture supernatant. Protein was purified by Ni-affinity chromatography following gel-filtration from cell culture supernatant.

SKU: 303-100 Categories: ,

Human GFRa-1 Chicken Polyclonal Antibody

The Human GFRa-1 Chicken Polyclonal Antibody is For Research Use Only

Immunogen: Human GFRa-1
Uniprot ID: P56159
Alternative Names: RET ligand 1, TGF-beta related neurotrophic factor receptor 1
Clonality: Chicken polyclonal
Class: IgY
Reactivity: Human GFRa-1 doesn’t cross-react with hGFRa-2, hGFRa-3, hGFRa-4
Application: ELISA, WB, IF
Purification: GFRa-1 affinity purified
Buffer: Concentrated ammonium sulphate in PBS pH 7.4
Concentration: 1 mg/ml
Unit Size: 100 µg
Related Products: Mono- and polyclonal antibodies to human growth factors and neural growth factor receptors.
Shipping: This product is shipped in non-frozen liquid form in ambient conditions.
Storage: Store at +4°C upon receipt. As product is (NH4)2SO4 precipitate, mix well by pipetting or vortexing prior use

Protocol: Western immunoblotting, recommended dilution 1:5 000 to 1:10 000; ELISA: 1:80 000 to 160 000, IF 1:1000 to 1:2000. Polyclonal antibody working titer has to be established practically for each particular antigen and assay format.

Background: Glial cell line-derived neurotrophic factor (GDNF) and neurturin (NTN) are two structurally related, potent neurotrophic factors that play key roles in the control of neuron survival and differentiation. The GFRa-1 is a member of the GDNF receptor family. It is a glycosylphosphatidylinositol (GPI)-linked cell surface receptor for both GDNF and NTN, and mediates activation of the RET tyrosine kinase receptor (www.ncbi.nlm.nih.gov/gene/2674).

Product Developed and Manufactured by Icosagen

Human GFRa-2 Chicken Polyclonal Antibody Isoptype IgY
Human GFRa-3 Chicken Polyclonal Antibody (Isoptype: IgY)
Human GFRa-4 Chicken Polyclonal Antibody Isoptype IgY

Additional Information

Figure 1:
Human GFRa-1 Chicken Polyclonal Antibody

Immunofluorescence  detection of human GFRα-1 expressed in U2OS cells. GFRα-1 was visualized using Chicken polyclonal antibody to human GFRα-1 (dilution 1:2000) and Goat anti-chicken IgY – H&L DyLight 550 (Abcam, ab96952) (dilution 1:200) as secondary antibody. For nuclear staining DAPI was used. ArrayScan VTI platform (Thermo Scientific) was used for image acquisition (10x objective). Composite picture was generated using pseudocolors green for GFRα-1 specific signal and blue for nuclei.

Figure 2:
Human GFRa-1 Chicken Polyclonal Antibody

Western-Blot detection of human GFRα-1, using Chicken polyclonal antibody to human GFRα-1. GFRα-1 was C-terminally His tagged. Protein was expressed by CHO cell culture. 100 ng of purified protein was loaded per lane. Line 1. Prestained protein ladder (Naxo 8003). Line 2. hGFRα-1. Primary antibody dilution 1:5000 was used. Rabbit anti-chicken antibody (Icosagen)  was used as secondary antibody.

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