Human F7 ELISA Assay Kit


Eagle Bioscience’s Human F7 ELISA Kit is intended for the measurement of Coagulation factor VI in cell culture supernatants, serum and plasma samples. The Human F7 ELISA Kit is for research use only and should not be used for diagnostic procedures.

Human F7 ELISA Assay Kit

The Human F7 ELISA Assay Kit is for Research Use Only

Size: 1×96 wells
Sensitivity: <20pg/ml
Incubation period: < 280 Minutes
Standard Range: 0.78 ng/ml – 50 ng/ml
Sample Type: Cell culture supernates, Serum and Plasma
Sample Size: 100µL
Alternative Names: Coagulation Factor VII

Assay Principle

The Eagle Biosciences Human F7 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Human F7 with a 96-well strip plate that is pre-coated with antibody specific for F7. The detection antibody is a biotinylated antibody specific for F7. The capture antibody is a monoclonal antibody from mouse and the detection antibody is a biotinylated polyclonal antibody from goat. The kit includes Human F7 protein as standards.

To measure Human F7, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Human F7 in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells’ readings against the standard curve to determine the concentration of Human F7 in the sample.

Related Products

Human F10 ELISA Kit
Human F11 ELISA Kit

Additional Information

Assay Background

Coagulation factor VII (EC, formerly known as proconvertin) is one of the proteins that causes blood to clot in the coagulation cascade, and in humans is coded for by the gene F7. This gene encodes coagulation factor VII which is a vitamin K-dependent factor essential for hemostasis. This factor circulates in the blood in a zymogen form, and is converted to an active form by either factor IXa, factor Xa, factor XIIa, or thrombin by minor proteolysis. Upon activation of the factor VII, a heavy chain containing a catalytic domain and a light chain containing 2 EGF-like domains are generated, and two chains are held together by a disulfide bond. In the presence of factor III and calcium ions, the activated factor then further activates the coagulation cascade by converting factor IX to factor IXa and/or factor X to factor Xa. Defects in this gene can cause coagulopathy. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar proteolytic processing to generate mature polypeptides.

Typical Standard Curve

Package Inserts

Please note: All documents above are for reference use only and should not be used in place of the documents included with this physical product. If digital copies are needed, please contact us.

Product Citations