Human F11 ELISA Assay Kit


Eagle Bioscience’s Human F11 ELISA Assay Kit is intended for the measurement of Human F10 in cell culture supernatants, serum and plasma samples. The Human F11 ELISA Assay Kit is for research use only and should not be used for diagnostic procedures.

Human F11 ELISA Assay Kit

The Human F11 ELISA Assay Kit is for Research Use Only

Size: 1×96 wells
Sensitivity: <20pg/ml
Incubation period: < 280 Minutes
Standard Range: 0.78 ng/ml – 50 ng/ml
Sample Type: Cell culture supernates, Serum and Plasma
Sample Size: 100µL
Alternative Names: Coagulation Factor XI

Assay Principle

The Eagle Biosciences Human F11 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Human F11 with a 96-well strip plate that is pre-coated with antibody specific for F11. The detection antibody is a biotinylated antibody specific for F11. The capture antibody is a monoclonal antibody from mouse and the detection antibody is a biotinylated polyclonal antibody from goat. The kit includes Human F11 protein as standards.

To measure Human F11, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Human F11 in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells’ readings against the standard curve to determine the concentration of Human F11 in the sample.

Related Products

Human F7 ELISA Assay Kit
Human F10 ELISA Assay Kit

Additional Information

Assay Background

Factor XI or plasma thromboplastin antecedent is the zymogen form of factor XIa, one of the enzymes of the coagulation cascade. Like many other coagulation factors, it is a serine protease. In humans, Factor XI is encoded by the F11 gene. This gene encodes coagulation factor XI of the blood coagulation cascade. This protein is present in plasma as a zymogen, which is a unique plasma coagulation enzyme because it exists as a homodimer consisting of two identical polypeptide chains linked by disulfide bonds. During activation of the plasma factor XI, an internal peptide bond is cleaved by factor XIIa (or XII) in each of the two chains, resulting in activated factor XIa, a serine protease composed of two heavy and two light chains held together by disulfide bonds. This activated plasma factor XI triggers the middle phase of the intrisic pathway of blood coagulation by activating factor IX. Defects in this factor lead to Rosenthal syndrome, a blood coagulation abnormality.

Typical Standard Curve

Package Inserts

Please note: All documents above are for reference use only and should not be used in place of the documents included with this physical product. If digital copies are needed, please contact us.

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