Anti-CCP ELISA Assay Kit
This item will be replacing CCP31-K01
Size: 1×96 wells
Sensitivity: 0.4 U/mL
Incubation Time: 1 hour 45 minutes
Sample Type: Serum
Sample Size: 100 µL
Alternative Names: Human Anti-CCP ELISA, Human CCP IgG ELISA, Cyclic Citrullinated Peptide
For Research Use Only
Preparation of Samples
The use of freshly collected serum from blood taken by venipuncture is recommended. The use of icteric, lipemic, hemolytic or bacterially contaminated samples should be avoided. Insoluble substances must be removed from the sample by centrifugation. Samples must not be thermally inactivated.
The samples must be diluted 1:101 (e. g. 10 μL + 1000 μL) with sample diluent and mixed thoroughly. Building of foam should be avoided.
Samples may be kept at 2 °C to 8 °C up to three days. Long-term storage requires -20 °C. Repeated freezing and thawing should be avoided. For multiple use, samples should be aliquoted and kept at -20 °C.
Rheumatoid Arthritis (RA) is most often diagnosed by the measurement of rheumatoid factors (RF). The rheumatoid factor is an antibody directed against the Fc-region of IgG. It appears mainly as IgM antibody but also as IgA or IgG subgroup. Rheumatoid factors are present in sera of 70 – 80 % of patients suffering from rheumatoid arthritis. But it is not specific for RA since it will also be found in healthy persons. The incidence
depends on the age: for young people it is 1 – 4 %, for older persons it may reach up to 25 %. The advantage of CCP antibodies is a higher sensitivity and specificity for the diagnosis of rheumatoid arthritis in comparison to the rheumatoid factors alone. Anti-CCP is often found at a very early state of the disease and it has a high predictive value for development of the disease.