Anti-CCP ELISA Assay Kit


The Anti-CCP ELISA Assay Kit is designed and validated for the quantitative determination of IgG antibodies against cyclic citrullinated peptides (CCP) in human serum. The Eagle Biosciences anti-CCP ELISA Assay Kit is for research use only and should not be used in diagnostic procedures.

Anti-CCP ELISA Assay Kit

This item will be replacing CCP31-K01

Size: 1×96 wells
Sensitivity: 0.4 U/mL
Incubation Time: 1 hour 45 minutes
Sample Type: Serum
Sample Size: 100 µL
Alternative Names: Human Anti-CCP ELISA, Human CCP IgG ELISA, Cyclic Citrullinated Peptide
For Research Use Only

Preparation of Samples

Sample Material
The use of freshly collected serum from blood taken by venipuncture is recommended. The use of icteric, lipemic, hemolytic or bacterially contaminated samples should be avoided. Insoluble substances must be removed from the sample by centrifugation. Samples must not be thermally inactivated.
Sample Dilution
The samples must be diluted 1:101 (e. g. 10 μL + 1000 μL) with sample diluent and mixed thoroughly. Building of foam should be avoided.
Sample Storage
Samples may be kept at 2 °C to 8 °C up to three days. Long-term storage requires -20 °C. Repeated freezing and thawing should be avoided. For multiple use, samples should be aliquoted and kept at -20 °C.

Assay Background

Rheumatoid Arthritis (RA) is most often diagnosed by the measurement of rheumatoid factors (RF). The rheumatoid factor is an antibody directed against the Fc-region of IgG. It appears mainly as IgM antibody but also as IgA or IgG subgroup. Rheumatoid factors are present in sera of 70 – 80 % of patients suffering from rheumatoid arthritis. But it is not specific for RA since it will also be found in healthy persons. The incidence
depends on the age: for young people it is 1 – 4 %, for older persons it may reach up to 25 %. The advantage of CCP antibodies is a higher sensitivity and specificity for the diagnosis of rheumatoid arthritis in comparison to the rheumatoid factors alone. Anti-CCP is often found at a very early state of the disease and it has a high predictive value for development of the disease.

Related Products

RF IgG (Rheumatoid Factor) ELISA Assay Kit
RF IgM (Rheumatoid Factor) ELISA Assay Kit
RF (Rheumatoid Factor) IgA ELISA Assay Kit

Additional Information

Assay Principle

The ELISA (Enzyme Linked Immunosorbent Assay) is an immunoassay for the determination of specific antibodies. The strips of the microtiter plate are coated with test-specific antigens. If antibodies are present in the patient´s sample, they bind to the antigens. A secondary antibody conjugated with the enzyme peroxidase detects the generated immune complex. A colorless substrate is converted into the colored product. The signal intensity of the reaction product is proportional to the antibody activity in the sample. After stopping the signal intensity of the reaction product is measured photometrically.



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