IMMUNOSCAN CCPlus ELISA Assay Kit
This item will be replacing 3665
Size: 1 × 96 wells
Standard Range: 25 – 3200 u/mL
Incubation Time: 2 hours
Sample Type: Serum
Sample Size: 100 µL
Alternative Names: Human Anti-CCP ELISA, Human CCP IgG ELISA, Cyclic Citrullinated Peptide
For Research Use Only
Assay Background
Rheumatoid Arthritis (RA) is one of the most common systemic autoimmune diseases. The aetiology of the disease, which affects up to 1-2% of the world population, is unknown. The diagnosis of RA depends primarily on clinical manifestation of the disease. The only serological test routinely used is the determination of the presence of rheumatoid factors (RF) in the serum. RF are antibodies directed to the constant region of immunoglobulins of the IgG class. However, these antibodies are also present in relatively high percentages in other autoimmune diseases, infections and in up to 15% of healthy individuals. Antibodies of a more specific nature have also been found in sera of RA patients. Anti-perinuclear factor (APF) antibodies are reported to be present in around 50% of RA patients with a specificity of over 70%. A number of cyclic synthetic peptides not related to filaggrin or other known proteins were described which are specifically recognized by autoantibodies in sera from RA patients. These peptides were subsequently used in an EIA for the detection of RA-specific autoantibodies. Clinical evaluation studies showed that the EIA was positive in a significant number of well-defined RA patient sera with an excellent specificity against disease controls. A diagnostic and prognostic value for the measurement of the anti Cyclic Citrullinated Peptides (anti-CCP) antibodies was found in relation to joint involvement and radiological damage in early RA. Anti-CCP antibodies can be detected years before the development of clinical symptoms. A prospective cohort study showed that 93% of the anti-CCP positive patients with undifferentiated arthritis finally developed rheumatoid arthritis, demonstrating the strong positive predictive value of these antibodies. The IMMUNOSCAN CCPlus® assay offered by Svar Life Science is based on highly purified synthetic peptides containing citrulline residues. This anti-CCP kit contains improved synthetic peptides selected on the basis of superior performance in the detection of RA autoantibodies.
Assay Principle
The anti-CCP antibody kit is based on an ELISA method. The test utilizes microtiter plate wells coated with citrullinated synthetic peptides (antigen). Diluted serum sample is applied to the wells and incubated. If specific antibodies are present, they will bind to the antigen in the wells. Unbound material is washed away and any bound antibody is detected by adding horse radish peroxidase (HRP) labelled anti-human IgG, followed by a second washing step and an incubation with substrate. The presence of reacting antibodies will result in the development of color, which is proportional to the quantity of bound antibody, and this is determined photometrically.
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