Human F10 ELISA Assay Kit


Eagle Bioscience’s Human F10 ELISA Kit is intended for the measurement of Human F10 in cell culture supernatants, serum and plasma samples. The Human F10 ELISA Kit is for research use only and should not be used for diagnostic procedures.

Human F10 ELISA Assay Kit

The Human F10 ELISA Assay Kit is for Research Use Only

Size: 1×96 wells
Sensitivity: <20pg/ml
Incubation period: < 280 Minutes
Standard Range: 0.78 ng/ml – 50 ng/ml
Sample Type: Cell culture supernates, Serum and Plasma
Sample Size: 100µL
Alternative Names: Coagulation Factor X

Assay Principle

The Eagle Biosciences Human F10 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Human F10 with a 96-well strip plate that is pre-coated with antibody specific for F10. The detection antibody is a biotinylated antibody specific for F10. The capture antibody is a monoclonal antibody from mouse and the detection antibody is a biotinylated polyclonal antibody from goat. The kit includes Human F10 protein as standards. To measure Human F10, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Human F10 in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells’ readings against the standard curve to determine the concentration of Human F10 in the sample.

Related Products

Human F7 ELISA Asasy Kit
Human F11 ELISA Assay Kit

Additional Information

Assay Background

This gene encodes the vitamin K-dependent coagulation factor X of the blood coagulation cascade. This factor undergoes multiple processing steps before its preproprotein is converted to a mature two-chain form by the excision of the tripeptide RKR. Two chains of the factor are held together by 1 or more disulfide bonds; the light chain contains 2 EGF-like domains, while the heavy chain contains the catalytic domain which is structurally homologous to those of the other hemostatic serine proteases. The mature factor is activated by the cleavage of the activation peptide by factor IXa (in the intrisic pathway), or by factor VIIa (in the extrinsic pathway). The activated factor then converts prothrombin to thrombin in the presence of factor Va, Ca+2, and phospholipid during blood clotting. Mutations of this gene result in factor X deficiency, a hemorrhagic condition of variable severity. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar proteolytic processing to generate mature polypeptides.

Typical Standard Curve

Human F10 ELISA Kit

Package Inserts

Please note: All documents above are for reference use only and should not be used in place of the documents included with this physical product. If digital copies are needed, please contact us.

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