High Sensitive IFN gamma ELISA Assay

Human ERBB4 ELISA Assay Kit

$540.00

The Eagle Bioscience’s Human ERBB4 ELISA Assay Kit is intended for the measurement of Receptor tyrosine-protein kinase erbB-4 in cell culture supernatants, serum and plasma samples. The Human ERBB4 ELISA Assay Kit is for research use only and should not be used for diagnostic procedures.

SKU: ERB31-K01 Categories: , Tags: , , , , , ,

Human ERBB4 ELISA Assay Kit

The Human ERBB4 ELISA Assay Kit is for Research Use Only

Size: 1×96 wells
Sensitivity: <10pg/ml
Incubation period: < 280 Minutes
Standard Range: 31.2 pg/ml – 2,000 pg/ml
Sample Type: Cell culture supernates, Serum and Plasma
Sample Size: 100µL
Alternative Names: Receptor tyrosine-protein kinase erbB-4

Assay Principle

Human ERBB4 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Human ERBB4 with a 96-well strip plate that is pre-coated with antibody specific for ERBB4. The detection antibody is a biotinylated antibody specific for ERBB4. The capture antibody is a monoclonal antibody from mouse and the detection antibody is a biotinylated polyclonal antibody from goat. The kit includes Human ERBB4 protein as standards.

To measure Human ERBB4, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Human ERBB4 in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells’ readings against the standard curve to determine the concentration of Human ERBB4 in the sample.

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Additional Information

Assay Background

Receptor tyrosine-protein kinase erbB-4 is an enzyme that in humans is encoded by the ERBB4 gene. This gene is a member of the Tyr protein kinase family and the epidermal growth factor receptor subfamily. It encodes a single-pass type I membrane protein with multiple cysteine rich domains, a transmembrane domain, a tyrosine kinase domain, a phosphotidylinositol-3 kinase binding site and a PDZ domain binding motif. The protein binds to and is activated by neuregulins and other factors and induces a variety of cellular responses including mitogenesis and differentiation. Multiple proteolytic events allow for the release of a cytoplasmic fragment and an extracellular fragment. Mutations in this gene have been associated with cancer. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized.

Typical Standard Curve

Package Inserts

Please note: All documents above are for reference use only and should not be used in place of the documents included with this physical product. If digital copies are needed, please contact us.

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